4UHT

Crystal structure of the DNA binding domain of CpxR from E. coli

Summary for 4UHT

• Entry DOI: 10.2210/pdb4uht/pdb
• Related: 4UHJ 4UHK 4UHS
• Descriptor: TRANSCRIPTIONAL REGULATORY PROTEIN CPXR, CHLORIDE ION (3 entities in total)
• Functional Keywords: transcription
• Biological source: ESCHERICHIA COLI
• Total number of polymer chains: 2
• Total formula weight: 23119.21

Authors

Mechaly, A.E.,Alzari, P.M.A. (deposition date: 2015-03-25, release date: 2016-04-13, Last modification date: 2024-05-08)

Primary citation

Mechaly, A.E.,Haouz, A.,Sassoon, N.,Buschiazzo, A.,Betton, J.M.,Alzari, P.M.
Conformational plasticity of the response regulator CpxR, a key player in Gammaproteobacteria virulence and drug-resistance.
J. Struct. Biol., 204:165-171, 2018

PubMed Abstract: The transcriptional regulator CpxR mediates an adaptive response to envelope stress, tightly linked to virulence and antibiotics resistance in several Gammaproteobacteria pathogens. In this work, we integrated crystallographic and small-angle X-ray scattering data to gain insights into the structure and conformational plasticity of CpxR from Escherichia coli. CpxR dimerizes through two alternative interaction surfaces. Moreover, widely different CpxR conformations coexist in solution, from compact to fully extended ones. The possible functional implications of these structural features are discussed.

PubMed: 30086390
DOI: 10.1016/j.jsb.2018.08.001

Experimental method

X-RAY DIFFRACTION (1.15 Å)