4UEI

Solution structure of the sterol carrier protein domain 2 of Helicoverpa armigera

Summary for 4UEI

• Entry DOI: 10.2210/pdb4uei/pdb
• Descriptor: STEROL CARRIER PROTEIN 2/3-OXOACYL-COA THIOLASE (1 entity in total)
• Functional Keywords: transferase, scp-2, insecticidal target
• Biological source: HELICOVERPA ARMIGERA (COTTON BOLLWORM)
• Total number of polymer chains: 1
• Total formula weight: 14051.94

Authors

Liu, X.,Ma, H.,Yan, X.,Hong, H.,Peng, J.,Peng, R. (deposition date: 2014-12-18, release date: 2015-12-30, Last modification date: 2024-05-15)

Primary citation

Ma, H.,Ma, Y.,Liu, X.,Dyer, D.H.,Xu, P.,Liu, K.,Lan, Q.,Hong, H.,Peng, J.,Peng, R.
NMR Structure and Function of Helicoverpa Armigera Sterol Carrier Protein-2, an Important Insecticidal Target from the Cotton Bollworm.
Sci.Rep., 5:18186-, 2015

PubMed Abstract: The cotton bollworm, Helicoverpa armigera, has developed strong resistance to many insecticides. Sterol Carrier Protein-2 (SCP-2) is an important non-specific lipid transfer protein in insects and appears to be a potential new target. In order to elucidate the structure and function of Helicoverpa armigera SCP-2 (HaSCP-2), NMR spectroscopy, docking simulations, mutagenesis and bioassays were performed. HaSCP-2 composed of five α-helices and four stranded β-sheets. The folds of α-helices and β-sheets interacted together to form a hydrophobic cavity with putative entrance and exit openings, which served as a tunnel for accommodating and transporting of lipids. Several sterols and fatty acids could interact with HaSCP-2 via important hydrophobic sites, which could be potential targets for insecticides. Mutagenesis experiments indicated Y51, F53, F89, F110, I117 and Q131 may be the key functional sites. HaSCP-2 showed high cholesterol binding activity and SCP-2 inhibitors (SCPIs) could inhibit the biological activity of HaSCP-2. SCPI-treated larvae at young stage showed a significant decrease of cholesterol uptake in vivo. Our study describes for the first time a NMR structure of SCP-2 in lepidopteran H. armigera and reveals its important function in cholesterol uptake, which facilitates the screening of effective insecticides targeting the insect cholesterol metabolism.

PubMed: 26655641
DOI: 10.1038/SREP18186

Experimental method

SOLUTION NMR