4UDV

Cryo-EM structure of TMV at 3.35 A resolution

4UDV の概要

• エントリーDOI: 10.2210/pdb4udv/pdb
• EMDBエントリー: 2842
• 分子名称: CAPSID PROTEIN, 5'-D(*GP*AP*AP)-3' (2 entities in total)
• 機能のキーワード: viral protein, direct electron detectors, single particle helical reconstruction, high resolution
• 由来する生物種: TOBACCO MOSAIC VIRUS; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 18464.09

構造登録者

Fromm, S.A.,Bharat, T.A.M.,Jakobi, A.J.,Hagen, W.J.H.,Sachse, C. (登録日: 2014-12-11, 公開日: 2014-12-31, 最終更新日: 2024-05-08)

主引用文献

Fromm, S.A.,Bharat, T.A.M.,Jakobi, A.J.,Hagen, W.J.H.,Sachse, C.
Seeing Tobacco Mosaic Virus Through Direct Electron Detectors.
J.Struct.Biol., 189:87-, 2015

PubMed Abstract: With the introduction of direct electron detectors (DED) to the field of electron cryo-microscopy, a wave of atomic-resolution structures has become available. As the new detectors still require comparative characterization, we have used tobacco mosaic virus (TMV) as a test specimen to study the quality of 3D image reconstructions from data recorded on the two direct electron detector cameras, K2 Summit and Falcon II. Using DED movie frames, we explored related image-processing aspects and compared the performance of micrograph-based and segment-based motion correction approaches. In addition, we investigated the effect of dose deposition on the atomic-resolution structure of TMV and show that radiation damage affects negative carboxyl chains first in a side-chain specific manner. Finally, using 450,000 asymmetric units and limiting the effects of radiation damage, we determined a high-resolution cryo-EM map at 3.35Å resolution. Here, we provide a comparative case study of highly ordered TMV recorded on different direct electron detectors to establish recording and processing conditions that enable structure determination up to 3.2Å in resolution using cryo-EM.

PubMed: 25528571
DOI: 10.1016/J.JSB.2014.12.002

実験手法

ELECTRON MICROSCOPY (3.35 Å)