4UDS

Crystal structure of MbdR regulator from Azoarcus sp. CIB

4UDS の概要

• エントリーDOI: 10.2210/pdb4uds/pdb
• 分子名称: MBDR REGULATOR (2 entities in total)
• 機能のキーワード: transcription, tetr family
• 由来する生物種: AZOARCUS SP. CIB
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24240.93

構造登録者

Liu, H.,Liu, H.X.,MacMahon, S.A.,Juarez, J.F.,Zamarro, M.T.,Eberlein, C.,Boll, M.,Carmona, M.,Diaz, E.,Naismith, J.H. (登録日: 2014-12-11, 公開日: 2014-12-24, 最終更新日: 2024-10-23)

主引用文献

Juarez, J.F.,Liu, H.,Zamarro, M.T.,Mcmahon, S.,Liu, H.,Naismith, J.H.,Eberlein, C.,Boll, M.,Carmona, M.,Diaz, E.
Unraveling the Specific Regulation of the Mbd Central Pathway for the Anaerobic Degradation of 3-Methylbenzoate.
J.Biol.Chem., 290:12165-, 2015

PubMed Abstract: The mbd cluster encodes the anaerobic degradation of 3-methylbenzoate in the β-proteobacterium Azoarcus sp. CIB. The specific transcriptional regulation circuit that controls the expression of the mbd genes was investigated. The PO, PB 1, and P3 R promoters responsible for the expression of the mbd genes, their cognate MbdR transcriptional repressor, as well as the MbdR operator regions (ATACN10GTAT) have been characterized. The three-dimensional structure of MbdR has been solved revealing a conformation similar to that of other TetR family transcriptional regulators. The first intermediate of the catabolic pathway, i.e. 3-methylbenzoyl-CoA, was shown to act as the inducer molecule. An additional MbdR-dependent promoter, PA, which contributes to the expression of the CoA ligase that activates 3-methylbenzoate to 3-methylbenzoyl-CoA, was shown to be necessary for an efficient induction of the mbd genes. Our results suggest that the mbd cluster recruited a regulatory system based on the MbdR regulator and its target promoters to evolve a distinct central catabolic pathway that is only expressed for the anaerobic degradation of aromatic compounds that generate 3-methylbenzoyl-CoA as the central metabolite. All these results highlight the importance of the regulatory systems in the evolution and adaptation of bacteria to the anaerobic degradation of aromatic compounds.

PubMed: 25795774
DOI: 10.1074/JBC.M115.637074

実験手法

X-RAY DIFFRACTION (1.76 Å)