4UCW

Structure of the T18V small subunit mutant of D. fructosovorans NiFe- hydrogenase

4UCW の概要

• エントリーDOI: 10.2210/pdb4ucw/pdb
• 関連するPDBエントリー: 4UCQ 4UCX
• 分子名称: HYDROGENASE (NIFE) SMALL SUBUNIT HYDA, NICKEL-DEPENDENT HYDROGENASE LARGE SUBUNIT, GLYCEROL, ... (9 entities in total)
• 機能のキーワード: oxidoreductase, nife-hydrogenase mutant, unready state
• 由来する生物種: DESULFOVIBRIO FRUCTOSIVORANS; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 272752.73

構造登録者

Abou-Hamdan, A.,Ceccaldi, P.,Lebrette, H.,Guttierez-Sanz, O.,Richaud, P.,Cournac, L.,Guigliarelli, B.,deLacey, A.L.,Leger, C.,Volbeda, A.,Burlat, B.,Dementin, S. (登録日: 2014-12-04, 公開日: 2015-02-18, 最終更新日: 2024-11-20)

主引用文献

Abou-Hamdan, A.,Ceccaldi, P.,Lebrette, H.,Gutierrez-Sanz, O.,Richaud, P.,Cournac, L.,Guigliarelli, B.,De Lacey, A.L.,Leger, C.,Volbeda, A.,Burlat, B.,Dementin, S.
A Threonine Stabilizes the Nic and Nir Catalytic Intermediates of [Nife]-Hydrogenase.
J.Biol.Chem., 290:8550-, 2015

PubMed Abstract: The heterodimeric [NiFe] hydrogenase from Desulfovibrio fructosovorans catalyzes the reversible oxidation of H2 into protons and electrons. The catalytic intermediates have been attributed to forms of the active site (NiSI, NiR, and NiC) detected using spectroscopic methods under potentiometric but non-catalytic conditions. Here, we produced variants by replacing the conserved Thr-18 residue in the small subunit with Ser, Val, Gln, Gly, or Asp, and we analyzed the effects of these mutations on the kinetic (H2 oxidation, H2 production, and H/D exchange), spectroscopic (IR, EPR), and structural properties of the enzyme. The mutations disrupt the H-bond network in the crystals and have a strong effect on H2 oxidation and H2 production turnover rates. However, the absence of correlation between activity and rate of H/D exchange in the series of variants suggests that the alcoholic group of Thr-18 is not necessarily a proton relay. Instead, the correlation between H2 oxidation and production activity and the detection of the NiC species in reduced samples confirms that NiC is a catalytic intermediate and suggests that Thr-18 is important to stabilize the local protein structure of the active site ensuring fast NiSI-NiC-NiR interconversions during H2 oxidation/production.

PubMed: 25666617
DOI: 10.1074/JBC.M114.630491

実験手法

X-RAY DIFFRACTION (2.3 Å)