4UC2

Crystal structure of translocator protein 18kDa (TSPO) from rhodobacter sphaeroides (A139T mutant) in P212121 space group

4UC2 の概要

• エントリーDOI: 10.2210/pdb4uc2/pdb
• 関連するPDBエントリー: 4UC1 4UC3
• 分子名称: TRANSLOCATOR PROTEIN TSPO, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, TETRAETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: mitochondria, transport, transmembrane protein, membrane protein
• 由来する生物種: Rhodobacter sphaeroides
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37959.05

構造登録者

Li, F.,Liu, J.,Zheng, Y.,Garavito, R.M.,Ferguson-Miller, S. (登録日: 2014-08-13, 公開日: 2015-02-04, 最終更新日: 2023-09-27)

主引用文献

Li, F.,Liu, J.,Zheng, Y.,Garavito, R.M.,Ferguson-Miller, S.
Crystal structures of translocator protein (TSPO) and mutant mimic of a human polymorphism.
Science, 347:555-558, 2015

PubMed Abstract: The 18-kilodalton translocator protein (TSPO), proposed to be a key player in cholesterol transport into mitochondria, is highly expressed in steroidogenic tissues, metastatic cancer, and inflammatory and neurological diseases such as Alzheimer's and Parkinson's. TSPO ligands, including benzodiazepine drugs, are implicated in regulating apoptosis and are extensively used in diagnostic imaging. We report crystal structures (at 1.8, 2.4, and 2.5 angstrom resolution) of TSPO from Rhodobacter sphaeroides and a mutant that mimics the human Ala(147)→Thr(147) polymorphism associated with psychiatric disorders and reduced pregnenolone production. Crystals obtained in the lipidic cubic phase reveal the binding site of an endogenous porphyrin ligand and conformational effects of the mutation. The three crystal structures show the same tightly interacting dimer and provide insights into the controversial physiological role of TSPO and how the mutation affects cholesterol binding.

PubMed: 25635101
DOI: 10.1126/science.1260590

実験手法

X-RAY DIFFRACTION (2.4 Å)