4UBG

Resting state of rat cysteine dioxygenase C93G variant

4UBG の概要

• エントリーDOI: 10.2210/pdb4ubg/pdb
• 関連するPDBエントリー: 4UBH
• 分子名称: Cysteine dioxygenase type 1, FE (II) ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: cysteine dioxygenase, non-heme mono-iron, cupin, cysteine to glycine substitution, oxidoreductase
• 由来する生物種: Rattus norvegicus (Rat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24475.86

構造登録者

Fellner, M.,Tchesnokov, E.P.,Jameson, G.N.,Wilbanks, S.M. (登録日: 2014-08-13, 公開日: 2014-11-26, 最終更新日: 2023-09-27)

主引用文献

Davies, C.G.,Fellner, M.,Tchesnokov, E.P.,Wilbanks, S.M.,Jameson, G.N.
The Cys-Tyr Cross-Link of Cysteine Dioxygenase Changes the Optimal pH of the Reaction without a Structural Change.
Biochemistry, 53:7961-7968, 2014

PubMed Abstract: Cysteine dioxygenase (CDO) is a non-heme monoiron enzyme with an unusual posttranslational modification in the proximity of the ferrous iron active site. This modification, a cysteine to tyrosine thioether bond, cross-links two β-strands of the β-barrel. We have investigated its role in catalysis through a combined crystallographic and kinetic approach. The C93G variant lacks the cross-link and shows little change in structure from that of the wild type, suggesting that the cross-link does not stabilize an otherwise unfavorable conformation. A pH-dependent kinetic study shows that both cross-linked and un-cross-linked CDO are active but the optimal pH decreases with the presence of the cross-link. This result reflects the effect of the thioether bond on the pKa of Y157 and this residue's role in catalysis. At higher pH values, kcat is also higher for the cross-linked form, extending the pH range of activity. We therefore propose that the cross-link also increases activity by controlling deleterious interactions involving the thiol/ate of C93.

PubMed: 25390690
DOI: 10.1021/bi501277a

実験手法

X-RAY DIFFRACTION (1.9 Å)