4UAJ

Crystal structure of NqrF in hexagonal space group

4UAJ の概要

• エントリーDOI: 10.2210/pdb4uaj/pdb
• 関連するPDBエントリー: 4U9U
• 分子名称: Na(+)-translocating NADH-quinone reductase subunit F, FLAVIN-ADENINE DINUCLEOTIDE, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: vibrio cholerae, sodium translocation, nqr, oxidoreductase
• 由来する生物種: Vibrio cholerae
• 細胞内の位置: Cell inner membrane ; Single-pass membrane protein : A5F5Y4
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33310.21

構造登録者

Fritz, G. (登録日: 2014-08-10, 公開日: 2015-08-05, 最終更新日: 2023-12-20)

主引用文献

Steuber, J.,Vohl, G.,Casutt, M.S.,Vorburger, T.,Diederichs, K.,Fritz, G.
Structure of the V. cholerae Na+-pumping NADH:quinone oxidoreductase.
Nature, 516:62-67, 2014

PubMed Abstract: NADH oxidation in the respiratory chain is coupled to ion translocation across the membrane to build up an electrochemical gradient. The sodium-translocating NADH:quinone oxidoreductase (Na(+)-NQR), a membrane protein complex widespread among pathogenic bacteria, consists of six subunits, NqrA, B, C, D, E and F. To our knowledge, no structural information on the Na(+)-NQR complex has been available until now. Here we present the crystal structure of the Na(+)-NQR complex at 3.5 Å resolution. The arrangement of cofactors both at the cytoplasmic and the periplasmic side of the complex, together with a hitherto unknown iron centre in the midst of the membrane-embedded part, reveals an electron transfer pathway from the NADH-oxidizing cytoplasmic NqrF subunit across the membrane to the periplasmic NqrC, and back to the quinone reduction site on NqrA located in the cytoplasm. A sodium channel was localized in subunit NqrB, which represents the largest membrane subunit of the Na(+)-NQR and is structurally related to urea and ammonia transporters. On the basis of the structure we propose a mechanism of redox-driven Na(+) translocation where the change in redox state of the flavin mononucleotide cofactor in NqrB triggers the transport of Na(+) through the observed channel.

PubMed: 25471880
DOI: 10.1038/nature14003

実験手法

X-RAY DIFFRACTION (2.7019 Å)