4U7E

The crystal structure of the complex of LIP5 NTD and IST1 MIM

4U7E の概要

• エントリーDOI: 10.2210/pdb4u7e/pdb
• 関連するPDBエントリー: 4U7I 4U7Y
• 分子名称: Vacuolar protein sorting-associated protein VTA1 homolog, IST1 homolog (3 entities in total)
• 機能のキーワード: complex, mim1, protein transport
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 21801.99

構造登録者

Guo, E.Z.,Xu, Z. (登録日: 2014-07-30, 公開日: 2015-02-11, 最終更新日: 2023-12-27)

主引用文献

Guo, E.Z.,Xu, Z.
Distinct Mechanisms of Recognizing Endosomal Sorting Complex Required for Transport III (ESCRT-III) Protein IST1 by Different Microtubule Interacting and Trafficking (MIT) Domains.
J.Biol.Chem., 290:8396-8408, 2015

PubMed Abstract: The endosomal sorting complex required for transport (ESCRT) machinery is responsible for membrane remodeling in a number of biological processes including multivesicular body biogenesis, cytokinesis, and enveloped virus budding. In mammalian cells, efficient abscission during cytokinesis requires proper function of the ESCRT-III protein IST1, which binds to the microtubule interacting and trafficking (MIT) domains of VPS4, LIP5, and Spartin via its C-terminal MIT-interacting motif (MIM). Here, we studied the molecular interactions between IST1 and the three MIT domain-containing proteins to understand the structural basis that governs pairwise MIT-MIM interaction. Crystal structures of the three molecular complexes revealed that IST1 binds to the MIT domains of VPS4, LIP5, and Spartin using two different mechanisms (MIM1 mode versus MIM3 mode). Structural comparison revealed that structural features in both MIT and MIM contribute to determine the specific binding mechanism. Within the IST1 MIM sequence, two phenylalanine residues were shown to be important in discriminating MIM1 versus MIM3 binding. These observations enabled us to deduce a preliminary binding code, which we applied to provide CHMP2A, a protein that normally only binds the MIT domain in the MIM1 mode, the additional ability to bind the MIT domain of Spartin in the MIM3 mode.

PubMed: 25657007
DOI: 10.1074/jbc.M114.607903

実験手法

X-RAY DIFFRACTION (1.6 Å)