4U4J
Crystal structure of Salmonella alpha-2-macroglobulin mutant Y1175G
Summary for 4U4J
| Entry DOI | 10.2210/pdb4u4j/pdb |
| Descriptor | Putative inner membrane lipoprotein (1 entity in total) |
| Functional Keywords | thioester, protease inhibitor, hydrolase inhibitor |
| Biological source | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
| Total number of polymer chains | 1 |
| Total formula weight | 179948.08 |
| Authors | Wong, S.G.,Dessen, A. (deposition date: 2014-07-23, release date: 2014-09-17, Last modification date: 2023-12-20) |
| Primary citation | Wong, S.G.,Dessen, A. Structure of a bacterial alpha 2-macroglobulin reveals mimicry of eukaryotic innate immunity. Nat Commun, 5:4917-4917, 2014 Cited by PubMed Abstract: Alpha-2-macroglobulins (A2Ms) are plasma proteins that trap and inhibit a broad range of proteases and are major components of the eukaryotic innate immune system. Surprisingly, A2M-like proteins were identified in pathogenically invasive bacteria and species that colonize higher eukaryotes. Bacterial A2Ms are located in the periplasm where they are believed to provide protection to the cell by trapping external proteases through a covalent interaction with an activated thioester. Here we report the crystal structures and characterization of Salmonella enterica ser. Typhimurium A2M in different states of thioester activation. The structures reveal thirteen domains whose arrangement displays high similarity to proteins involved in eukaryotic immune defence. A structural lock mechanism maintains the stability of the buried thioester, a requirement for its protease-trapping activity. These findings indicate that bacteria have developed a rudimentary innate immune system whose mechanism mimics that of eukaryotes. PubMed: 25221932DOI: 10.1038/ncomms5917 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.9 Å) |
Structure validation
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