4U3P

Octameric RNA duplex co-crystallized with strontium(II)chloride

4U3P の概要

• エントリーDOI: 10.2210/pdb4u3p/pdb
• 関連するPDBエントリー: 4U3L 4U3O
• 分子名称: RNA (5'-R(*UP*CP*GP*UP*AP*CP*GP*A)-3'), STRONTIUM ION (3 entities in total)
• 機能のキーワード: rna, duplex, di- and trivalent metal ions
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5228.36

構造登録者

Schaffer, M.F.,Spingler, B.,Schnabl, J.,Peng, G.,Olieric, V.,Sigel, R.K.O. (登録日: 2014-07-22, 公開日: 2015-07-29, 最終更新日: 2023-12-20)

主引用文献

Schaffer, M.F.,Peng, G.,Spingler, B.,Schnabl, J.,Wang, M.,Olieric, V.,Sigel, R.K.
The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations.
Int J Mol Sci, 17:-, 2016

PubMed Abstract: Due to the polyanionic nature of RNA, the principles of charge neutralization and electrostatic condensation require that cations help to overcome the repulsive forces in order for RNA to adopt a three-dimensional structure. A precise structural knowledge of RNA-metal ion interactions is crucial to understand the mechanism of metal ions in the catalytic or regulatory activity of RNA. We solved the crystal structure of an octameric RNA duplex in the presence of the di- and trivalent metal ions Ca(2+), Mn(2+), Co(2+), Cu(2+), Sr(2+), and Tb(3+). The detailed investigation reveals a unique innersphere interaction to uracil and extends the knowledge of the influence of metal ions for conformational changes in RNA structure. Furthermore, we could demonstrate that an accurate localization of the metal ions in the X-ray structures require the consideration of several crystallographic and geometrical parameters as well as the anomalous difference map.

PubMed: 27355942
DOI: 10.3390/ijms17070988

実験手法

X-RAY DIFFRACTION (1.866 Å)