4TYW

DEAD-box helicase Mss116 bound to ssRNA and ADP-BeF

4TYW の概要

• エントリーDOI: 10.2210/pdb4tyw/pdb
• 関連するPDBエントリー: 4TYN 4TYY 4TZ0 4TZ6
• 分子名称: ATP-dependent RNA helicase MSS116, mitochondrial, RNA (5'-R(P*AP*AP*AP*AP*AP*AP*A)-3'), MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: dead-box, rna helicase, hydrolase, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Mitochondrion matrix : P15424
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60587.00

構造登録者

Mallam, A.L.,Sidote, D.J.,Lambowitz, A.M. (登録日: 2014-07-09, 公開日: 2014-12-31, 最終更新日: 2023-09-27)

主引用文献

Mallam, A.L.,Sidote, D.J.,Lambowitz, A.M.
Molecular insights into RNA and DNA helicase evolution from the determinants of specificity for a DEAD-box RNA helicase.
Elife, 3:e04630-e04630, 2014

PubMed Abstract: How different helicase families with a conserved catalytic 'helicase core' evolved to function on varied RNA and DNA substrates by diverse mechanisms remains unclear. In this study, we used Mss116, a yeast DEAD-box protein that utilizes ATP to locally unwind dsRNA, to investigate helicase specificity and mechanism. Our results define the molecular basis for the substrate specificity of a DEAD-box protein. Additionally, they show that Mss116 has ambiguous substrate-binding properties and interacts with all four NTPs and both RNA and DNA. The efficiency of unwinding correlates with the stability of the 'closed-state' helicase core, a complex with nucleotide and nucleic acid that forms as duplexes are unwound. Crystal structures reveal that core stability is modulated by family-specific interactions that favor certain substrates. This suggests how present-day helicases diversified from an ancestral core with broad specificity by retaining core closure as a common catalytic mechanism while optimizing substrate-binding interactions for different cellular functions.

PubMed: 25497230
DOI: 10.7554/eLife.04630

実験手法

X-RAY DIFFRACTION (2.197 Å)