4TX1

The crystal structure of carbohydrate acetylesterase family member from Sinorhizobium meliloti

4TX1 の概要

• エントリーDOI: 10.2210/pdb4tx1/pdb
• 分子名称: Esterase (2 entities in total)
• 機能のキーワード: sgnh-hydrolase, acetylesterase, sgnh-family, hydrolase
• 由来する生物種: Sinorhizobium meliloti 1021 (Ensifer meliloti)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 73054.67

構造登録者

Kim, K.,Kim, S.S.,Pandian, R.,Ngo, T.D. (登録日: 2014-07-02, 公開日: 2014-12-24, 最終更新日: 2023-11-08)

主引用文献

Kim, K.,Ryu, B.H.,Kim, S.S.,An, D.R.,Ngo, T.D.,Pandian, R.,Kim, K.K.,Kim, T.D.
Structural and biochemical characterization of a carbohydrate acetylesterase from Sinorhizobium meliloti 1021.
Febs Lett., 589:117-122, 2015

PubMed Abstract: In many microorganisms, carbohydrate acetylesterases remove the acetyl groups from various types of carbohydrates. Sm23 from Sinorhizobium meliloti is a putative member of carbohydrate esterase family 3 (CE3) in the CAZy classification system. Here, we determined the crystal structure of Sm23 at 1.75 Å resolution and investigated functional properties using biochemical methods. Furthermore, immobilized Sm23 exhibited improved stability compared with soluble Sm23, which can be used for the design of plant cell wall degrading-systems.

PubMed: 25436419
DOI: 10.1016/j.febslet.2014.11.033

実験手法

X-RAY DIFFRACTION (1.75 Å)