4TV7

Crystal structure of Bacillus subtilis GabR at 2.05 Angstroms resolution

4TV7 の概要

• エントリーDOI: 10.2210/pdb4tv7/pdb
• 分子名称: HTH-type transcriptional regulatory protein GabR (2 entities in total)
• 機能のキーワード: transcriptional regulator, transcription
• 由来する生物種: Bacillus subtilis 168
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 221872.86

構造登録者

Goto, M.,Okuda, K.,Yoshimura, T. (登録日: 2014-06-26, 公開日: 2014-12-24, 最終更新日: 2025-04-09)

主引用文献

Okuda, K.,Kato, S.,Ito, T.,Shiraki, S.,Kawase, Y.,Goto, M.,Kawashima, S.,Hemmi, H.,Fukada, H.,Yoshimura, T.
Role of the aminotransferase domain in Bacillus subtilis GabR, a pyridoxal 5'-phosphate-dependent transcriptional regulator
Mol. Microbiol., 95:245-257, 2015

PubMed Abstract: MocR/GabR family proteins are widely distributed prokaryotic transcriptional regulators containing pyridoxal 5'-phosphate (PLP), a coenzyme form of vitamin B6. The Bacillus subtilis GabR, probably the most extensively studied MocR/GabR family protein, consists of an N-terminal DNA-binding domain and a PLP-binding C-terminal domain that has a structure homologous to aminotransferases. GabR suppresses transcription of gabR and activates transcription of gabT and gabD, which encode γ-aminobutyrate (GΑΒΑ) aminotransferase and succinate semialdehyde dehydrogenase, respectively, in the presence of PLP and GABA. In this study, we examined the mechanism underlying GabR-mediated gabTD transcription with spectroscopic, crystallographic and thermodynamic studies, focusing on the function of the aminotransferase domain. Spectroscopic studies revealed that GABA forms an external aldimine with the PLP in the aminotransferase domain. Isothermal calorimetry demonstrated that two GabR molecules bind to the 51-bp DNA fragment that contains the GabR-binding region. GABA minimally affected ΔG(binding) upon binding of GabR to the DNA fragment but greatly affected the contributions of ΔH and ΔS to ΔG(binding). GABA forms an external aldimine with PLP and causes a conformational change in the aminotransferase domain, and this change likely rearranges GabR binding to the promoter and thus activates gabTD transcription.

PubMed: 25388514
DOI: 10.1111/mmi.12861

実験手法

X-RAY DIFFRACTION (2.05 Å)