4TU1

Structure of Toxoplasma gondii fructose 1,6 bisphosphate aldolase

4TU1 の概要

• エントリーDOI: 10.2210/pdb4tu1/pdb
• 分子名称: Fructose-1,6-bisphosphate aldolase, GLYCEROL (3 entities in total)
• 機能のキーワード: aldolase, f16bp, invasion, toxoplasma, glideosome, structural genomics, psi-2, protein structure initiative, seattle structural genomics center for infectious disease, ssgcid, lyase
• 由来する生物種: Toxoplasma gondii
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 154308.56

構造登録者

Boucher, L.E.,Bosch, J.,Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2014-06-23, 公開日: 2014-09-03, 最終更新日: 2023-09-27)

主引用文献

Boucher, L.E.,Bosch, J.
Structure of Toxoplasma gondii fructose-1,6-bisphosphate aldolase.
Acta Crystallogr.,Sect.F, 70:1186-1192, 2014

PubMed Abstract: The apicomplexan parasite Toxoplasma gondii must invade host cells to continue its lifecycle. It invades different cell types using an actomyosin motor that is connected to extracellular adhesins via the bridging protein fructose-1,6-bisphosphate aldolase. During invasion, aldolase serves in the role of a structural bridging protein, as opposed to its normal enzymatic role in the glycolysis pathway. Crystal structures of the homologous Plasmodium falciparum fructose-1,6-bisphosphate aldolase have been described previously. Here, T. gondii fructose-1,6-bisphosphate aldolase has been crystallized in space group P22121, with the biologically relevant tetramer in the asymmetric unit, and the structure has been determined via molecular replacement to a resolution of 2.0 Å. An analysis of the quality of the model and of the differences between the four chains in the asymmetric unit and a comparison between the T. gondii and P. falciparum aldolase structures is presented.

PubMed: 25195889
DOI: 10.1107/S2053230X14017087

実験手法

X-RAY DIFFRACTION (2 Å)