4TSS

TOXIC SHOCK SYNDROME TOXIN-1: TETRAGONAL P4(1)2(1)2 CRYSTAL FORM

Summary for 4TSS

• Entry DOI: 10.2210/pdb4tss/pdb
• Descriptor: TOXIC SHOCK SYNDROME TOXIN-1, ZINC ION (2 entities in total)
• Functional Keywords: toxin, superantigen
• Biological source: Staphylococcus aureus
• Cellular location: Secreted: P06886
• Total number of polymer chains: 1
• Total formula weight: 22169.18

Authors

Prasad, G.S.,Radhakrishnan, R.,Mitchell, D.T.,Earhart, C.A.,Dinges, M.M.,Cook, W.J.,Schlivert, P.M.,Ohlendorf, D.H. (deposition date: 1996-12-11, release date: 1997-12-24, Last modification date: 2024-05-22)

Primary citation

Prasad, G.S.,Radhakrishnan, R.,Mitchell, D.T.,Earhart, C.A.,Dinges, M.M.,Cook, W.J.,Schlievert, P.M.,Ohlendorf, D.H.
Refined structures of three crystal forms of toxic shock syndrome toxin-1 and of a tetramutant with reduced activity.
Protein Sci., 6:1220-1227, 1997

PubMed Abstract: The structure of toxic shock syndrome toxin-1 (TSST-1), the causative agent in toxic shock syndrome, has been determined in three crystal forms. The three structural models have been refined to R-factors of 0.154, 0.150, and 0.198 at resolutions of 2.05 A, 2.90 A, and 2.75 A, respectively. One crystal form of TSST-1 contains a zinc ion bound between two symmetry-related molecules. Although not required for biological activity, zinc dramatically potentiates the mitogenicity of TSST-1 at very low concentrations. In addition, the structure of the tetramutant TSST-1H [T69I, Y80W, E132K, I140T], which is nonmitogenic and does not amplify endotoxin shock, has been determined and refined in a fourth crystal form (R-factor = 0.173 to 1.9 A resolution).

PubMed: 9194182

Experimental method

X-RAY DIFFRACTION (2.75 Å)