4TQM

Structural basis of specific recognition of non-reducing terminal N-acetylglucosamine by an Agrocybe aegerita lection

4TQM の概要

• エントリーDOI: 10.2210/pdb4tqm/pdb
• 関連するPDBエントリー: 4TQJ 4TQK
• 分子名称: Lectin 2, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose, ... (4 entities in total)
• 機能のキーワード: complex, lectin, glcnac, sugar binding protein
• 由来する生物種: Agrocybe aegerita (Black poplar mushroom)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46398.35

構造登録者

Hu, Y.L.,Ren, X.M.,Li, D.F.,Jiang, S.,Lan, X.Q.,Sun, H.,Wang, D.C. (登録日: 2014-06-11, 公開日: 2015-06-03, 最終更新日: 2024-03-20)

主引用文献

Ren, X.M.,Li, D.F.,Jiang, S.,Lan, X.Q.,Hu, Y.,Sun, H.,Wang, D.C.
Structural Basis of Specific Recognition of Non-Reducing Terminal N-Acetylglucosamine by an Agrocybe aegerita Lectin.
Plos One, 10:e0129608-e0129608, 2015

PubMed Abstract: O-linked N-acetylglucosaminylation (O-GlcNAcylation) is a reversible post-translational modification that plays essential roles in many cellular pathways. Research in this field, however, is hampered by the lack of suitable probes to identify, accumulate, and purify the O-GlcNAcylated proteins. We have previously reported the identification of a lectin from the mushroom Agrocybe aegerita, i.e., Agrocybe aegerita lectin 2, or AAL2, that could bind terminal N-acetylglucosamine with higher affinities and specificity than other currently used probes. In this paper, we report the crystal structures of AAL2 and its complexes with GlcNAc and GlcNAcβ1-3Galβ1-4GlcNAc and reveal the structural basis of GlcNAc recognition by AAL2 and residues essential for the binding of terminal N-acetylglucosamine. Study on AAL2 may enable us to design a protein probe that can be used to identify and purify O-GlcNAcylated proteins more efficiently.

PubMed: 26114302
DOI: 10.1371/journal.pone.0129608

実験手法

X-RAY DIFFRACTION (2 Å)