4TPU

CRYSTAL STRUCTURE OF FERREDOXIN-DEPENDENT DISULFIDE REDUCTASE FROM METHANOSARCINA ACETIVORANS

4TPU の概要

• エントリーDOI: 10.2210/pdb4tpu/pdb
• 分子名称: Rubredoxin, SULFATE ION, IRON/SULFUR CLUSTER, ... (8 entities in total)
• 機能のキーワード: ftr-like protein, disulfide reductase, electron transport
• 由来する生物種: Methanosarcina acetivorans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21635.17

構造登録者

Kumar, A.K.,Yennawar, H.P.,Yennawar, N.H.,Ferry, J.G. (登録日: 2014-06-09, 公開日: 2015-05-13, 最終更新日: 2024-10-09)

主引用文献

Kumar, A.K.,Kumar, R.S.,Yennawar, N.H.,Yennawar, H.P.,Ferry, J.G.
Structural and Biochemical Characterization of a Ferredoxin:Thioredoxin Reductase-like Enzyme from Methanosarcina acetivorans.
Biochemistry, 54:3122-3128, 2015

PubMed Abstract: Bioinformatics analyses predict the distribution in nature of several classes of diverse disulfide reductases that evolved from an ancestral plant-type ferredoxin:thioredoxin reductase (FTR) catalytic subunit to meet a variety of ecological needs. Methanosarcina acetivorans is a methane-producing species from the domain Archaea predicted to encode an FTR-like enzyme with two domains, one resembling the FTR catalytic subunit and the other containing a rubredoxin-like domain replacing the variable subunit of present-day FTR enzymes. M. acetivorans is of special interest as it was recently proposed to have evolved at the time of the end-Permian extinction and to be largely responsible for the most severe biotic crisis in the fossil record by converting acetate to methane. The crystal structure and biochemical characteristics were determined for the FTR-like enzyme from M. acetivorans, here named FDR (ferredoxin disulfide reductase). The results support a role for the rubredoxin-like center of FDR in transfer of electrons from ferredoxin to the active-site [Fe₄S₄] cluster adjacent to a pair of redox-active cysteines participating in reduction of disulfide substrates. A mechanism is proposed for disulfide reduction similar to one of two mechanisms previously proposed for the plant-type FTR. Overall, the results advance the biochemical and evolutionary understanding of the FTR-like family of enzymes and the conversion of acetate to methane that is an essential link in the global carbon cycle and presently accounts for most of this greenhouse gas that is biologically generated.

PubMed: 25915695
DOI: 10.1021/acs.biochem.5b00137

実験手法

X-RAY DIFFRACTION (2.355 Å)