4TPJ
Selectivity mechanism of a bacterial homologue of the human drug peptide transporters PepT1 and PepT2
Summary for 4TPJ
| Entry DOI | 10.2210/pdb4tpj/pdb |
| Related | 4tpg 4tph |
| Descriptor | Proton:oligopeptide symporter POT family, ALA-ALA-ALA, DODECYL-BETA-D-MALTOSIDE, ... (4 entities in total) |
| Functional Keywords | membrane protein, secondary active transporter, complex |
| Biological source | Shewanella oneidensis More |
| Total number of polymer chains | 4 |
| Total formula weight | 116508.06 |
| Authors | Guettou, F.,Quistgaard, E.,Raba, M.,Moberg, P.,Low, C.,Nordlund, P. (deposition date: 2014-06-07, release date: 2014-07-09, Last modification date: 2023-12-20) |
| Primary citation | Guettou, F.,Quistgaard, E.M.,Raba, M.,Moberg, P.,Low, C.,Nordlund, P. Selectivity mechanism of a bacterial homolog of the human drug-peptide transporters PepT1 and PepT2. Nat.Struct.Mol.Biol., 21:728-, 2014 Cited by PubMed Abstract: Peptide transporters of the PepT family have key roles in the transport of di- and tripeptides across membranes as well as in the absorption of orally administered drugs in the small intestine. We have determined structures of a PepT transporter from Shewanella oneidensis (PepT(So2)) in complex with three different peptides. The peptides bind in a large cavity lined by residues that are highly conserved in human PepT1 and PepT2. The bound peptides adopt extended conformations with their N termini clamped into a conserved polar pocket. A positively charged patch allows differential interactions with the C-terminal carboxylates of di- and tripeptides. Here we identify three pockets for peptide side chain interactions, and our binding studies define differential roles of these pockets for the recognition of different subtypes of peptide side chains. PubMed: 25064511DOI: 10.1038/nsmb.2860 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.201 Å) |
Structure validation
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