4TM7

Crystal structure of 6-phosphogluconolactonase from Mycobacterium smegmatis N131D mutant soaked with CuSO4

4TM7 の概要

• エントリーDOI: 10.2210/pdb4tm7/pdb
• 関連するPDBエントリー: 4TM8
• 分子名称: 6-phosphogluconolactonase, 1,2-ETHANEDIOL, SULFATE ION, ... (7 entities in total)
• 機能のキーワード: rossmann fold, hydrolase
• 由来する生物種: Mycobacterium smegmatis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28313.82

構造登録者

Fujieda, N.,Stuttfeld, E.,Maier, T. (登録日: 2014-05-31, 公開日: 2015-06-03, 最終更新日: 2024-03-20)

主引用文献

Fujieda, N.,Schatti, J.,Stuttfeld, E.,Ohkubo, K.,Maier, T.,Fukuzumi, S.,Ward, T.R.
Enzyme repurposing of a hydrolase as an emergent peroxidase upon metal binding.
Chem Sci, 6:4060-4065, 2015

PubMed Abstract: As an alternative to Darwinian evolution relying on catalytic promiscuity, a protein may acquire auxiliary function upon metal binding, thus providing it with a novel catalytic machinery. Here we show that addition of cupric ions to a 6-phosphogluconolactonase bearing a putative metal binding site leads to the emergence of peroxidase activity ( 7.8 × 10 s, 1.1 × 10 M). Both X-ray crystallographic and EPR data of the copper-loaded enzyme reveal a bis-histidine coordination site, located within a shallow binding pocket capable of accommodating the -dianisidine substrate.

PubMed: 29218172
DOI: 10.1039/c5sc01065a

実験手法

X-RAY DIFFRACTION (1.39 Å)