4TKR

Native-SAD phasing for ThiT from Listeria monocytogenes serovar.

4TKR の概要

• エントリーDOI: 10.2210/pdb4tkr/pdb
• 分子名称: Thiamine transporter thia, THIAMINE DIPHOSPHATE, 2-O-octyl-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: membrane protein, transporter, native-sad phasing, multiple crystals, low energy, structural genomics, psi-biology, new york consortium on membrane protein structure, nycomps
• 由来する生物種: Listeria monocytogenes
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 53394.85

構造登録者

Guo, Y.,Liu, Q.,Hendrickson, W.A.,New York Consortium on Membrane Protein Structure (NYCOMPS) (登録日: 2014-05-27, 公開日: 2014-06-18, 最終更新日: 2023-12-27)

主引用文献

Liu, Q.,Guo, Y.,Chang, Y.,Cai, Z.,Assur, Z.,Mancia, F.,Greene, M.I.,Hendrickson, W.A.
Multi-crystal native SAD analysis at 6 keV.
Acta Crystallogr.,Sect.D, 70:2544-2557, 2014

PubMed Abstract: Anomalous diffraction signals from typical native macromolecules are very weak, frustrating their use in de novo structure determination. Here, native SAD procedures are described to enhance signal to noise in anomalous diffraction by using multiple crystals in combination with synchrotron X-rays at 6 keV. Increased anomalous signals were obtained at 6 keV compared with 7 keV X-ray energy, which was used for previous native SAD analyses. A feasibility test of multi-crystal-based native SAD phasing was performed at 3.2 Å resolution for a known tyrosine protein kinase domain, and real-life applications were made to two novel membrane proteins at about 3.0 Å resolution. The three applications collectively serve to validate the robust feasibility of native SAD phasing at lower energy.

PubMed: 25286840
DOI: 10.1107/S1399004714013376

実験手法

X-RAY DIFFRACTION (3.0023 Å)