4S3L

Crystal Structure of major pilin protein PitB from type II pilus of Streptococcus pneumoniae

4S3L の概要

• エントリーDOI: 10.2210/pdb4s3l/pdb
• 分子名称: Major Pilin Protein (2 entities in total)
• 機能のキーワード: major pilin, type ii pilus, virulence, cnab, igg fold, igg-rev fold, pilin protein, cell adhesion
• 由来する生物種: Streptococcus pneumoniae (strain Taiwan19F-14)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 74395.78

構造登録者

Shaik, M.M.,Dessen, A.,Di Guilmi, A.M. (登録日: 2015-02-10, 公開日: 2015-07-29, 最終更新日: 2024-11-27)

主引用文献

Shaik, M.M.,Lombardi, C.,Maragno Trindade, D.,Fenel, D.,Schoehn, G.,Di Guilmi, A.M.,Dessen, A.
A Structural Snapshot of Type II Pilus Formation in Streptococcus pneumoniae.
J.Biol.Chem., 290:22581-22592, 2015

PubMed Abstract: Pili are fibrous appendages expressed on the surface of a vast number of bacterial species, and their role in surface adhesion is important for processes such as infection, colonization, andbiofilm formation. The human pathogen Streptococcus pneumoniae expresses two different types of pili, PI-1 and PI-2, both of which require the concerted action of structural proteins and sortases for their polymerization. The type PI-1 streptococcal pilus is a complex, well studied structure, but the PI-2 type, present in a number of invasive pneumococcal serotypes, has to date remained less well understood. The PI-2 pilus consists of repeated units of a single protein, PitB, whose covalent association is catalyzed by cognate sortase SrtG-1 and partner protein SipA. Here we report the high resolution crystal structures of PitB and SrtG1 and use molecular modeling to visualize a "trapped" 1:1 complex between the two molecules. X-ray crystallography and electron microscopy reveal that the pneumococcal PI-2 backbone fiber is formed by PitB monomers associated in head-to-tail fashion and that short, flexible fibers can be formed even in the absence of coadjuvant proteins. These observations, obtained with a simple pilus biosynthetic system, are likely to be applicable to other fiber formation processes in a variety of Gram-positive organisms.

PubMed: 26198632
DOI: 10.1074/jbc.M115.647834

実験手法

X-RAY DIFFRACTION (2.8 Å)