4RYN

Crystal structure of BcTSPO, type1 monomer

4RYN の概要

• エントリーDOI: 10.2210/pdb4ryn/pdb
• 関連するPDBエントリー: 4RYI 4RYJ 4RYM 4RYO
• 分子名称: Integral membrane protein, [(Z)-octadec-9-enyl] (2R)-2,3-bis(oxidanyl)propanoate, DODECYL-ALPHA-D-MALTOSIDE, ... (6 entities in total)
• 機能のキーワード: structural genomics, psi-biology, protein structure initiative, new york consortium on membrane protein structure, nycomps, receptor, membrane protein
• 由来する生物種: Bacillus cereus
• 細胞内の位置: Cell membrane ; Multi-pass membrane protein : Q81BL7
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25503.60

構造登録者

Guo, Y.,Liu, Q.,Hendrickson, W.A.,New York Consortium on Membrane Protein Structure (NYCOMPS) (登録日: 2014-12-15, 公開日: 2015-02-11, 最終更新日: 2023-09-20)

主引用文献

Guo, Y.,Kalathur, R.C.,Liu, Q.,Kloss, B.,Bruni, R.,Ginter, C.,Kloppmann, E.,Rost, B.,Hendrickson, W.A.
Protein structure. Structure and activity of tryptophan-rich TSPO proteins.
Science, 347:551-555, 2015

PubMed Abstract: Translocator proteins (TSPOs) bind steroids and porphyrins, and they are implicated in many human diseases, for which they serve as biomarkers and therapeutic targets. TSPOs have tryptophan-rich sequences that are highly conserved from bacteria to mammals. Here we report crystal structures for Bacillus cereus TSPO (BcTSPO) down to 1.7 Å resolution, including a complex with the benzodiazepine-like inhibitor PK11195. We also describe BcTSPO-mediated protoporphyrin IX (PpIX) reactions, including catalytic degradation to a previously undescribed heme derivative. We used structure-inspired mutations to investigate reaction mechanisms, and we showed that TSPOs from Xenopus and man have similar PpIX-directed activities. Although TSPOs have been regarded as transporters, the catalytic activity in PpIX degradation suggests physiological importance for TSPOs in protection against oxidative stress.

PubMed: 25635100
DOI: 10.1126/science.aaa1534

実験手法

X-RAY DIFFRACTION (2.01 Å)