4RW5

Structural insights into substrate binding of brown spider venom class II phospholipases D

4RW5 の概要

• エントリーDOI: 10.2210/pdb4rw5/pdb
• 関連するPDBエントリー: 4RW3
• 分子名称: Phospholipase D LiSicTox-alphaIA1bii, MAGNESIUM ION, PALMITIC ACID, ... (7 entities in total)
• 機能のキーワード: tim-barrel fold, hydrolase
• 由来する生物種: Loxosceles intermedia (Brown spider)
• 細胞内の位置: Secreted : P0CE82
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34404.00

構造登録者

Coronado, M.A.,Ullah, A.,da Silva, L.S.,Chaves-Moreira, D.,Vuitika, L.,Chaim, O.M.,Veiga, S.S.,Chahine, J.,Murakami, M.T.,Arni, R.K. (登録日: 2014-12-01, 公開日: 2015-06-03, 最終更新日: 2024-11-06)

主引用文献

Coronado, M.A.,Ullah, A.,da Silva, L.S.,Chaves-Moreira, D.,Vuitika, L.,Chaim, O.M.,Veiga, S.S.,Chahine, J.,Murakami, M.T.,Arni, R.K.
Structural Insights into Substrate Binding of Brown Spider Venom Class II Phospholipases D.
Curr Protein Pept Sci, 16:768-774, 2015

PubMed Abstract: Phospholipases D (PLDs), the major dermonecrotic factors from brown spider venoms, trigger a range of biological reactions both in vitro and in vivo. Despite their clinical relevance in loxoscelism, structural data is restricted to the apo-form of these enzymes, which has been instrumental in understanding the functional differences between the class I and II spider PLDs. The crystal structures of the native class II PLD from Loxosceles intermedia complexed with myo-inositol 1-phosphate and the inactive mutant H12A complexed with fatty acids indicate the existence of a strong ligand-dependent conformation change of the highly conserved aromatic residues, Tyr 223 and Trp225 indicating their roles in substrate binding. These results provided insights into the structural determinants for substrate recognition and binding by class II PLDs.

PubMed: 25961401

実験手法

X-RAY DIFFRACTION (1.64 Å)