4RW2

Hen egg-white lysozyme structure from a spent-beam experiment at LCLS: refocused beam

4RW2 の概要

• エントリーDOI: 10.2210/pdb4rw2/pdb
• 関連するPDBエントリー: 4RW1
• 分子名称: Lysozyme C, SODIUM ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Gallus gallus (bantam,chickens)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14389.60

構造登録者

Boutet, S.,Foucar, L.,Barends, T.,Doak, R.B.,Koglin, J.E.,Messerschmidt, M.,Nass, K.,Schlichting, I.,Shoeman, R.,Williams, G.J. (登録日: 2014-12-01, 公開日: 2015-05-20, 最終更新日: 2024-11-20)

主引用文献

Boutet, S.,Foucar, L.,Barends, T.R.,Botha, S.,Doak, R.B.,Koglin, J.E.,Messerschmidt, M.,Nass, K.,Schlichting, I.,Seibert, M.M.,Shoeman, R.L.,Williams, G.J.
Characterization and use of the spent beam for serial operation of LCLS.
J.SYNCHROTRON RADIAT., 22:634-643, 2015

PubMed Abstract: X-ray free-electron laser sources such as the Linac Coherent Light Source offer very exciting possibilities for unique research. However, beam time at such facilities is very limited and in high demand. This has led to significant efforts towards beam multiplexing of various forms. One such effort involves re-using the so-called spent beam that passes through the hole in an area detector after a weak interaction with a primary sample. This beam can be refocused into a secondary interaction region and used for a second, independent experiment operating in series. The beam profile of this refocused beam was characterized for a particular experimental geometry at the Coherent X-ray Imaging instrument at LCLS. A demonstration of this multiplexing capability was performed with two simultaneous serial femtosecond crystallography experiments, both yielding interpretable data of sufficient quality to produce electron density maps.

PubMed: 25931079
DOI: 10.1107/S1600577515004002

実験手法

X-RAY DIFFRACTION (2.3 Å)