4RVY

Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3)

Replaces:  4Q54

Summary for 4RVY

• Entry DOI: 10.2210/pdb4rvy/pdb
• Descriptor: Photosystem II protein D1 1, Photosystem II reaction center protein K, Photosystem II reaction center protein L, ... (34 entities in total)
• Functional Keywords: photosystem ii, time resolved, free electron laser, electron transport, membrane, oxidoreductase
• Biological source: Thermosynechococcus elongatus; More
• Total number of polymer chains: 38
• Total formula weight: 704848.02

Authors

Kupitz, C.,Basu, S.,Grotjohann, I.,Fromme, R.,Zatsepin, N.,Rendek, K.N.,Hunter, M.,Shoeman, R.L.,White, T.A.,Wang, D.,James, D.,Yang, J.-H.,Cobb, D.E.,Reeder, B.,Sierra, R.G.,Liu, H.,Barty, A.,Aquila, A.,Deponte, D.,Kirian, R.,Bari, S.,Bergkamp, J.J.,Beyerlein, K.,Bogan, M.J.,Caleman, C.,Chao, T.-C.,Conrad, C.E.,Davis, K.M.,Fleckenstein, H.,Galli, L.,Hau-Riege, S.P.,Kassemeyer, S.,Laksmono, H.,Liang, M.,Lomb, L.,Marchesini, S.,Martin, A.V.,Messerschmidt, M.,Milathianaki, D.,Nass, K.,Ros, A.,Roy-Chowdhury, S.,Schmidt, K.,Seibert, M.,Steinbrener, J.,Stellato, F.,Yan, L.,Yoon, C.,Moore, T.A.,Moore, A.L.,Pushkar, Y.,Williams, G.J.,Boutet, S.,Doak, R.B.,Weierstall, U.,Frank, M.,Chapman, H.N.,Spence, J.C.H.,Fromme, P. (deposition date: 2014-11-29, release date: 2015-11-04, Last modification date: 2023-09-20)

Primary citation

Kupitz, C.,Basu, S.,Grotjohann, I.,Fromme, R.,Zatsepin, N.A.,Rendek, K.N.,Hunter, M.S.,Shoeman, R.L.,White, T.A.,Wang, D.,James, D.,Yang, J.H.,Cobb, D.E.,Reeder, B.,Sierra, R.G.,Liu, H.,Barty, A.,Aquila, A.L.,Deponte, D.,Kirian, R.A.,Bari, S.,Bergkamp, J.J.,Beyerlein, K.R.,Bogan, M.J.,Caleman, C.,Chao, T.C.,Conrad, C.E.,Davis, K.M.,Fleckenstein, H.,Galli, L.,Hau-Riege, S.P.,Kassemeyer, S.,Laksmono, H.,Liang, M.,Lomb, L.,Marchesini, S.,Martin, A.V.,Messerschmidt, M.,Milathianaki, D.,Nass, K.,Ros, A.,Roy-Chowdhury, S.,Schmidt, K.,Seibert, M.,Steinbrener, J.,Stellato, F.,Yan, L.,Yoon, C.,Moore, T.A.,Moore, A.L.,Pushkar, Y.,Williams, G.J.,Boutet, S.,Doak, R.B.,Weierstall, U.,Frank, M.,Chapman, H.N.,Spence, J.C.,Fromme, P.
Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser.
Nature, 513:261-265, 2014

PubMed Abstract: Photosynthesis, a process catalysed by plants, algae and cyanobacteria converts sunlight to energy thus sustaining all higher life on Earth. Two large membrane protein complexes, photosystem I and II (PSI and PSII), act in series to catalyse the light-driven reactions in photosynthesis. PSII catalyses the light-driven water splitting process, which maintains the Earth's oxygenic atmosphere. In this process, the oxygen-evolving complex (OEC) of PSII cycles through five states, S0 to S4, in which four electrons are sequentially extracted from the OEC in four light-driven charge-separation events. Here we describe time resolved experiments on PSII nano/microcrystals from Thermosynechococcus elongatus performed with the recently developed technique of serial femtosecond crystallography. Structures have been determined from PSII in the dark S1 state and after double laser excitation (putative S3 state) at 5 and 5.5 Å resolution, respectively. The results provide evidence that PSII undergoes significant conformational changes at the electron acceptor side and at the Mn4CaO5 core of the OEC. These include an elongation of the metal cluster, accompanied by changes in the protein environment, which could allow for binding of the second substrate water molecule between the more distant protruding Mn (referred to as the 'dangler' Mn) and the Mn3CaOx cubane in the S2 to S3 transition, as predicted by spectroscopic and computational studies. This work shows the great potential for time-resolved serial femtosecond crystallography for investigation of catalytic processes in biomolecules.

PubMed: 25043005
DOI: 10.1038/nature13453

Experimental method

X-RAY DIFFRACTION (5.5 Å)