4RUF

Human K2P4.1 (TRAAAK) potassium channel, W262S mutant

4RUF の概要

• エントリーDOI: 10.2210/pdb4ruf/pdb
• 関連するPDBエントリー: 4RUE
• 分子名称: Potassium channel subfamily K member 4, POTASSIUM ION (2 entities in total)
• 機能のキーワード: traak potassium ion channel, metal transport
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell membrane ; Multi-pass membrane protein : Q9NYG8
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 67207.93

構造登録者

Lolicato, M.,Minor, D.L.Jr. (登録日: 2014-11-19, 公開日: 2014-12-17, 最終更新日: 2024-11-06)

主引用文献

Lolicato, M.,Riegelhaupt, P.M.,Arrigoni, C.,Clark, K.A.,Minor, D.L.
Transmembrane Helix Straightening and Buckling Underlies Activation of Mechanosensitive and Thermosensitive K2P Channels.
Neuron, 84:1198-1212, 2014

PubMed Abstract: Mechanical and thermal activation of ion channels is central to touch, thermosensation, and pain. The TRAAK/TREK K(2P) potassium channel subfamily produces background currents that alter neuronal excitability in response to pressure, temperature, signaling lipids, and anesthetics. How such diverse stimuli control channel function is unclear. Here we report structures of K(2P)4.1 (TRAAK) bearing C-type gate-activating mutations that reveal a tilting and straightening of the M4 inner transmembrane helix and a buckling of the M2 transmembrane helix. These conformational changes move M4 in a direction opposite to that in classical potassium channel activation mechanisms and open a passage lateral to the pore that faces the lipid bilayer inner leaflet. Together, our findings uncover a unique aspect of K(2P) modulation, indicate a means for how the K(2P) C-terminal cytoplasmic domain affects the C-type gate which lies ∼40Å away, and suggest how lipids and bilayer inner leaflet deformations may gate the channel.

PubMed: 25500157
DOI: 10.1016/j.neuron.2014.11.017

実験手法

X-RAY DIFFRACTION (3.4 Å)