4RSI

Yeast Smc2-Smc4 hinge domain with extended coiled coils

4RSI の概要

• エントリーDOI: 10.2210/pdb4rsi/pdb
• 関連するPDBエントリー: 4RSJ
• 分子名称: Structural maintenance of chromosomes protein 2, Structural maintenance of chromosomes protein 4, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: smc hinge domain with coiled coil, chromosomal condensation, cell cycle
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Nucleus: P38989 Q12267
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 90804.02

構造登録者

Soh, Y.M.,Shin, H.C.,Oh, B.H. (登録日: 2014-11-08, 公開日: 2014-12-31, 最終更新日: 2024-02-28)

主引用文献

Soh, Y.M.,Burmann, F.,Shin, H.C.,Oda, T.,Jin, K.S.,Toseland, C.P.,Kim, C.,Lee, H.,Kim, S.J.,Kong, M.S.,Durand-Diebold, M.L.,Kim, Y.G.,Kim, H.M.,Lee, N.K.,Sato, M.,Oh, B.H.,Gruber, S.
Molecular Basis for SMC Rod Formation and Its Dissolution upon DNA Binding.
Mol.Cell, 57:290-303, 2015

PubMed Abstract: SMC condensin complexes are central modulators of chromosome superstructure in all branches of life. Their SMC subunits form a long intramolecular coiled coil, which connects a constitutive "hinge" dimerization domain with an ATP-regulated "head" dimerization module. Here, we address the structural arrangement of the long coiled coils in SMC complexes. We unequivocally show that prokaryotic Smc-ScpAB, eukaryotic condensin, and possibly also cohesin form rod-like structures, with their coiled coils being closely juxtaposed and accurately anchored to the hinge. Upon ATP-induced binding of DNA to the hinge, however, Smc switches to a more open configuration. Our data suggest that a long-distance structural transition is transmitted from the Smc head domains to regulate Smc-ScpAB's association with DNA. These findings uncover a conserved architectural theme in SMC complexes, provide a mechanistic basis for Smc's dynamic engagement with chromosomes, and offer a molecular explanation for defects in Cornelia de Lange syndrome.

PubMed: 25557547
DOI: 10.1016/j.molcel.2014.11.023

実験手法

X-RAY DIFFRACTION (2.9 Å)