4RMO

Crystal Structure of the CptIN Type III Toxin-Antitoxin System from Eubacterium rectale

4RMO の概要

• エントリーDOI: 10.2210/pdb4rmo/pdb
• 分子名称: CptN Toxin, RNA (45-MER), CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: protein-rna complex, pseudoknot, rna twist, toxin-antitoxin, type iii, bacteriophage resistance, toxin-rna complex, toxin/rna
• 由来する生物種: Eubacterium rectale DSM 17629; 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 267950.58

構造登録者

Rao, F.,Voss, J.E.,Short, F.L.,Luisi, B.F. (登録日: 2014-10-21, 公開日: 2015-09-30, 最終更新日: 2024-11-06)

主引用文献

Rao, F.,Short, F.L.,Voss, J.E.,Blower, T.R.,Orme, A.L.,Whittaker, T.E.,Luisi, B.F.,Salmond, G.P.
Co-evolution of quaternary organization and novel RNA tertiary interactions revealed in the crystal structure of a bacterial protein-RNA toxin-antitoxin system.
Nucleic Acids Res., 43:9529-9540, 2015

PubMed Abstract: Genes encoding toxin-antitoxin (TA) systems are near ubiquitous in bacterial genomes and they play key roles in important aspects of bacterial physiology, including genomic stability, formation of persister cells under antibiotic stress, and resistance to phage infection. The CptIN locus from Eubacterium rectale is a member of the recently-discovered Type III class of TA systems, defined by a protein toxin suppressed by direct interaction with a structured RNA antitoxin. Here, we present the crystal structure of the CptIN protein-RNA complex to 2.2 Å resolution. The structure reveals a new heterotetrameric quaternary organization for the Type III TA class, and the RNA antitoxin bears a novel structural feature of an extended A-twist motif within the pseudoknot fold. The retention of a conserved ribonuclease active site as well as traits normally associated with TA systems, such as plasmid maintenance, implicates a wider functional role for Type III TA systems. We present evidence for the co-variation of the Type III component pair, highlighting a distinctive evolutionary process in which an enzyme and its substrate co-evolve.

PubMed: 26350213
DOI: 10.1093/nar/gkv868

実験手法

X-RAY DIFFRACTION (2.2 Å)