4RLV

Crystal Structure of AnkB 24 Ankyrin Repeats in Complex with AnkR Autoinhibition Segment

4RLV の概要

• エントリーDOI: 10.2210/pdb4rlv/pdb
• 関連するPDBエントリー: 4RLY
• 分子名称: Ankyrin-1, Ankyrin-2, SULFATE ION (2 entities in total)
• 機能のキーワード: ank repeat, protein-protein interaction, structural protein
• 由来する生物種: Mus musculus (mouse, human); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton : Q01484
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 98876.09

構造登録者

Wei, Z.,Wang, C.,Zhang, M. (登録日: 2014-10-18, 公開日: 2014-11-26, 最終更新日: 2024-11-27)

主引用文献

Wang, C.,Wei, Z.,Chen, K.,Ye, F.,Yu, C.,Bennett, V.,Zhang, M.
Structural basis of diverse membrane target recognitions by ankyrins.
Elife, 3:e04353-, 2014

PubMed Abstract: Ankyrin adaptors together with their spectrin partners coordinate diverse ion channels and cell adhesion molecules within plasma membrane domains and thereby promote physiological activities including fast signaling in the heart and nervous system. Ankyrins specifically bind to numerous membrane targets through their 24 ankyrin repeats (ANK repeats), although the mechanism for the facile and independent evolution of these interactions has not been resolved. Here we report the structures of ANK repeats in complex with an inhibitory segment from the C-terminal regulatory domain and with a sodium channel Nav1.2 peptide, respectively, showing that the extended, extremely conserved inner groove spanning the entire ANK repeat solenoid contains multiple target binding sites capable of accommodating target proteins with very diverse sequences via combinatorial usage of these sites. These structures establish a framework for understanding the evolution of ankyrins' membrane targets, with implications for other proteins containing extended ANK repeat domains.

PubMed: 25383926
DOI: 10.7554/eLife.04353

実験手法

X-RAY DIFFRACTION (3.4945 Å)