4RLR

Structure of monoheme cytochrome PccH from Geobacter sulfurreducens

4RLR の概要

• エントリーDOI: 10.2210/pdb4rlr/pdb
• 分子名称: Cytochrome c, 1 heme-binding site, PROTOPORPHYRIN IX CONTAINING FE, ZINC ION, ... (6 entities in total)
• 機能のキーワード: novel monoheme cytochrome, electrode, electron transport
• 由来する生物種: Geobacter sulfurreducens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15870.68

構造登録者

Pokkuluri, P.R.,Salgueiro, C.A. (登録日: 2014-10-17, 公開日: 2015-04-01, 最終更新日: 2024-02-28)

主引用文献

Dantas, J.M.,Campelo, L.M.,Duke, N.E.,Salgueiro, C.A.,Pokkuluri, P.R.
The structure of PccH from Geobacter sulfurreducens - a novel low reduction potential monoheme cytochrome essential for accepting electrons from an electrode.
Febs J., 282:2215-2231, 2015

PubMed Abstract: The structure of cytochrome c (GSU3274) designated as PccH from Geobacter sulfurreducens was determined at a resolution of 2.0 Å. PccH is a small (15 kDa) cytochrome containing one c-type heme, found to be essential for the growth of G. sulfurreducens with respect to accepting electrons from graphite electrodes poised at -300 mV versus standard hydrogen electrode. with fumarate as the terminal electron acceptor. The structure of PccH is unique among the monoheme cytochromes described to date. The structural fold of PccH can be described as forming two lobes with the heme sandwiched in a cleft between the two lobes. In addition, PccH has a low reduction potential of -24 mV at pH 7, which is unusual for monoheme cytochromes. Based on difference in structure, together with sequence phylogenetic analysis, we propose that PccH can be regarded as a first characterized example of a new subclass of class I monoheme cytochromes. The low reduction potential of PccH may enable the protein to be redox active at the typically negative potential ranges encountered by G. sulfurreducens. Because PccH is predicted to be located in the periplasm of this bacterium, it could not be involved in the first step of accepting electrons from the electrode but is very likely involved in the downstream electron transport events in the periplasm.

PubMed: 25786707
DOI: 10.1111/febs.13269

実験手法

X-RAY DIFFRACTION (2.002 Å)