4RL8

Crystal structure of the COG4313 outer membrane channel from Pseudomonas putida F1

4RL8 の概要

• エントリーDOI: 10.2210/pdb4rl8/pdb
• 分子名称: Protein involved in meta-pathway of phenol degradation-like protein, (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: beta barrel, channel, bacterial outer membrane, membrane protein
• 由来する生物種: Pseudomonas putida
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 130561.80

構造登録者

van den Berg, B. (登録日: 2014-10-16, 公開日: 2015-07-29, 最終更新日: 2024-02-28)

主引用文献

Berg, B.v.,Bhamidimarri, S.P.,Winterhalter, M.
Crystal structure of a COG4313 outer membrane channel.
Sci Rep, 5:11927-11927, 2015

PubMed Abstract: COG4313 proteins form a large and widespread family of outer membrane channels and have been implicated in the uptake of a variety of hydrophobic molecules. Structure-function studies of this protein family have so far been hampered by a lack of structural information. Here we present the X-ray crystal structure of Pput2725 from the biodegrader Pseudomonas putida F1, a COG4313 channel of unknown function, using data to 2.3 Å resolution. The structure shows a 12-stranded barrel with an N-terminal segment preceding the first β-strand occluding the lumen of the barrel. Single channel electrophysiology and liposome swelling experiments suggest that while the narrow channel visible in the crystal structure does allow passage of ions and certain small molecules in vitro, Pput2725 is unlikely to function as a channel for hydrophilic molecules. Instead, the presence of bound detergent molecules inside the barrel suggests that Pput2725 mediates uptake of hydrophobic molecules. Sequence alignments and the locations of highly conserved residues suggest the presence of a dynamic lateral opening through which hydrophobic molecules might gain entry into the cell. Our results provide the basis for structure-function studies of COG4313 family members with known function, such as the SphA sphingosine uptake channel of Pseudomonas aeruginosa.

PubMed: 26149193
DOI: 10.1038/srep11927

実験手法

X-RAY DIFFRACTION (2.3 Å)