4RIE

Landomycin Glycosyltransferase LanGT2

4RIE の概要

• エントリーDOI: 10.2210/pdb4rie/pdb
• 分子名称: Glycosyl transferase homolog (2 entities in total)
• 機能のキーワード: gt fold, glycosyltransferase, transferase
• 由来する生物種: Streptomyces cyanogenus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 80812.21

構造登録者

Tam, H.K.,Gerhardt, S.,Breit, B.,Bechthold, A.,Einsle, O. (登録日: 2014-10-06, 公開日: 2015-01-28, 最終更新日: 2023-09-20)

主引用文献

Tam, H.K.,Harle, J.,Gerhardt, S.,Rohr, J.,Wang, G.,Thorson, J.S.,Bigot, A.,Lutterbeck, M.,Seiche, W.,Breit, B.,Bechthold, A.,Einsle, O.
Structural Characterization of O- and C-Glycosylating Variants of the Landomycin Glycosyltransferase LanGT2.
Angew.Chem.Int.Ed.Engl., 54:2811-2815, 2015

PubMed Abstract: The structures of the O-glycosyltransferase LanGT2 and the engineered, C-C bond-forming variant LanGT2S8Ac show how the replacement of a single loop can change the functionality of the enzyme. Crystal structures of the enzymes in complex with a nonhydrolyzable nucleotide-sugar analogue revealed that there is a conformational transition to create the binding sites for the aglycon substrate. This induced-fit transition was explored by molecular docking experiments with various aglycon substrates.

PubMed: 25581707
DOI: 10.1002/anie.201409792

実験手法

X-RAY DIFFRACTION (2.162 Å)