4RI6

Crystal structure of poplar glutathione transferase F1

4RI6 の概要

• エントリーDOI: 10.2210/pdb4ri6/pdb
• 関連するPDBエントリー: 4RI7
• 分子名称: Phi class glutathione transferase GSTF1, GLUTATHIONE, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (4 entities in total)
• 機能のキーワード: glutathione transferase fold, transferase
• 由来する生物種: Populus tremula x Populus tremuloides
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49708.58

構造登録者

Pegeot, H.,Koh, C.S.,Didierjean, C.,Rouhier, N. (登録日: 2014-10-05, 公開日: 2015-01-21, 最終更新日: 2023-09-20)

主引用文献

Pegeot, H.,Koh, C.S.,Petre, B.,Mathiot, S.,Duplessis, S.,Hecker, A.,Didierjean, C.,Rouhier, N.
The poplar Phi class glutathione transferase: expression, activity and structure of GSTF1.
Front Plant Sci, 5:712-712, 2014

PubMed Abstract: Glutathione transferases (GSTs) constitute a superfamily of enzymes with essential roles in cellular detoxification and secondary metabolism in plants as in other organisms. Several plant GSTs, including those of the Phi class (GSTFs), require a conserved catalytic serine residue to perform glutathione (GSH)-conjugation reactions. Genomic analyses revealed that terrestrial plants have around ten GSTFs, eight in the Populus trichocarpa genome, but their physiological functions and substrates are mostly unknown. Transcript expression analyses showed a predominant expression of all genes both in reproductive (female flowers, fruits, floral buds) and vegetative organs (leaves, petioles). Here, we show that the recombinant poplar GSTF1 (PttGSTF1) possesses peroxidase activity toward cumene hydroperoxide and GSH-conjugation activity toward model substrates such as 2,4-dinitrochlorobenzene, benzyl and phenetyl isothiocyanate, 4-nitrophenyl butyrate and 4-hydroxy-2-nonenal but interestingly not on previously identified GSTF-class substrates. In accordance with analytical gel filtration data, crystal structure of PttGSTF1 showed a canonical dimeric organization with bound GSH or 2-(N-morpholino)ethanesulfonic acid molecules. The structure of these protein-substrate complexes allowed delineating the residues contributing to both the G and H sites that form the active site cavity. In sum, the presence of GSTF1 transcripts and proteins in most poplar organs especially those rich in secondary metabolites such as flowers and fruits, together with its GSH-conjugation activity and its documented stress-responsive expression suggest that its function is associated with the catalytic transformation of metabolites and/or peroxide removal rather than with ligandin properties as previously reported for other GSTFs.

PubMed: 25566286
DOI: 10.3389/fpls.2014.00712

実験手法

X-RAY DIFFRACTION (1.523 Å)