4RI2

Crystal structure of the photoprotective protein PsbS from spinach

4RI2 の概要

• エントリーDOI: 10.2210/pdb4ri2/pdb
• 関連するPDBエントリー: 4RI3
• 分子名称: Photosystem II 22 kDa protein, chloroplastic, nonyl beta-D-glucopyranoside, CHLOROPHYLL A, ... (5 entities in total)
• 機能のキーワード: transmembrane helices, photo protection, pigment binding, membrane protein
• 由来する生物種: Spinacia oleracea (Spinach)
• 細胞内の位置: Plastid, chloroplast thylakoid membrane; Multi-pass membrane protein: Q02060
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47416.10

構造登録者

Fan, M.,Li, M.,Chang, W. (登録日: 2014-10-05, 公開日: 2015-08-12, 最終更新日: 2024-02-28)

主引用文献

Fan, M.,Li, M.,Liu, Z.,Cao, P.,Pan, X.,Zhang, H.,Zhao, X.,Zhang, J.,Chang, W.
Crystal structures of the PsbS protein essential for photoprotection in plants.
Nat.Struct.Mol.Biol., 22:729-735, 2015

PubMed Abstract: The photosystem II protein PsbS has an essential role in qE-type nonphotochemical quenching, which protects plants from photodamage under excess light conditions. qE is initiated by activation of PsbS by low pH, but the mechanism of PsbS action remains elusive. Here we report the low-pH crystal structures of PsbS from spinach in its free form and in complex with the qE inhibitor N,N'-dicyclohexylcarbodiimide (DCCD), revealing that PsbS adopts a unique folding pattern, and, unlike other members of the light-harvesting-complex superfamily, it is a noncanonical pigment-binding protein. Structural and biochemical evidence shows that both active and inactive PsbS form homodimers in the thylakoid membranes, and DCCD binding disrupts the lumenal intermolecular hydrogen bonds of the active PsbS dimer. Activation of PsbS by low pH during qE may involve a conformational change associated with altered lumenal intermolecular interactions of the PsbS dimer.

PubMed: 26258636
DOI: 10.1038/nsmb.3068

実験手法

X-RAY DIFFRACTION (2.35 Å)