4RH4

Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing

4RH4 の概要

• エントリーDOI: 10.2210/pdb4rh4/pdb
• 関連するPDBエントリー: 1PAZ 1PZA 1PZC 3NYK
• 分子名称: Pseudoazurin, ZINC ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: sad, beta-sandwich, divalent metal-ion, metalloprotein, metal binding protein
• 由来する生物種: Alcaligenes faecalis
• 細胞内の位置: Periplasm: P04377
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13641.05

構造登録者

Gessmann, R.,Petratos, K. (登録日: 2014-10-01, 公開日: 2015-01-14, 最終更新日: 2024-02-28)

主引用文献

Gessmann, R.,Papadovasilaki, M.,Drougkas, E.,Petratos, K.
Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing.
Acta Crystallogr F Struct Biol Commun, 71:19-23, 2015

PubMed Abstract: The copper(II) centre of the blue copper protein pseudoazurin from Alcaligenes faecalis has been substituted by zinc(II) via denaturing the protein, chelation and removal of copper and refolding the apoprotein, followed by the addition of an aqueous solution of ZnCl2. Vapour-diffusion experiments produced colourless hexagonal crystals (space group P65), which when cryocooled had unit-cell parameters a=b=49.01, c=98.08 Å. Diffraction data collected at 100 K using a copper sealed tube were phased by the weak anomalous signal of five S atoms and one Zn atom. The structure was fitted manually and refined to 1.6 Å resolution. The zinc-substituted protein exhibits similar overall geometry to the native structure with copper. Zn2+ binds more strongly to its four ligand atoms (His40 Nδ1, Cys78 Sγ, His81 Nδ1 and Met86 Sδ) and retains the tetrahedral arrangement, although the structure is less distorted than the native copper protein.

PubMed: 25615962
DOI: 10.1107/S2053230X14025552

実験手法

X-RAY DIFFRACTION (1.6 Å)