4RGZ
Crystal structure of recombinant prolidase from Thermococcus sibiricus at P21221 spacegroup
Summary for 4RGZ
| Entry DOI | 10.2210/pdb4rgz/pdb |
| Related | 4FKC |
| Descriptor | Xaa-Pro aminopeptidase, ZINC ION, PHOSPHATE ION, ... (4 entities in total) |
| Functional Keywords | aminopeptidase, dipeptidases, hydrolase |
| Biological source | Thermococcus sibiricus MM 739 |
| Total number of polymer chains | 4 |
| Total formula weight | 166157.42 |
| Authors | Timofeev, V.I.,Korgenevsky, D.A.,Gorbacheva, M.A.,Boyko, K.M.,Slutsky, E.,Rakitina, T.V.,Lipkin, A.V.,Popov, V.O. (deposition date: 2014-10-01, release date: 2015-08-12, Last modification date: 2024-02-28) |
| Primary citation | Timofeev, V.,Slutskaya, E.,Gorbacheva, M.,Boyko, K.,Rakitina, T.,Korzhenevskiy, D.,Lipkin, A.,Popov, V. Structure of recombinant prolidase from Thermococcus sibiricus in space group P21221. Acta Crystallogr.,Sect.F, 71:951-957, 2015 Cited by PubMed Abstract: The crystal structure of recombinant prolidase from Thermococcus sibiricus was determined by X-ray diffraction at a resolution of 2.6 Å and was found to contain a tetramer in the asymmetric unit. A protein crystal grown in microgravity using the counter-diffusion method was used for X-ray studies. The crystal belonged to space group P21221, with unit-cell parameters a = 97.60, b = 123.72, c = 136.52 Å, α = β = γ = 90°. The structure was refined to an Rcryst of 22.1% and an Rfree of 29.6%. The structure revealed flexible folding of the N-terminal domain of the protein as well as high variability in the positions of the bound metal ions. The coordinates of the resulting model were deposited in the Protein Data Bank as entry 4rgz. PubMed: 26249680DOI: 10.1107/S2053230X15009498 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.6 Å) |
Structure validation
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