4RGA

Phage 1358 receptor binding protein in complex with the trisaccharide GlcNAc-Galf-GlcOMe

4RGA の概要

• エントリーDOI: 10.2210/pdb4rga/pdb
• 関連するPDBエントリー: 4L92 4L97 4L99
• 分子名称: Phage 1358 receptor binding protein (ORF20), 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-beta-D-galactofuranose-(1-6)-methyl alpha-D-glucopyranoside (3 entities in total)
• 機能のキーワード: alpha/beta protein, phage receptor binding protein, lactococcus lactis smq388, viral protein
• 由来する生物種: Lactococcus phage 1358
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 87803.09

構造登録者

Spinelli, S.,Mccabe, O.,Farenc, C.,Tremblay, D.,Blangy, S.,Oscarson, S.,Moineau, S.,Cambillau, C. (登録日: 2014-09-29, 公開日: 2015-05-20, 最終更新日: 2023-09-20)

主引用文献

McCabe, O.,Spinelli, S.,Farenc, C.,Labbe, M.,Tremblay, D.,Blangy, S.,Oscarson, S.,Moineau, S.,Cambillau, C.
The targeted recognition of Lactococcus lactis phages to their polysaccharide receptors.
Mol.Microbiol., 96:875-886, 2015

PubMed Abstract: Each phage infects a limited number of bacterial strains through highly specific interactions of the receptor-binding protein (RBP) at the tip of phage tail and the receptor at the bacterial surface. Lactococcus lactis is covered with a thin polysaccharide pellicle (hexasaccharide repeating units), which is used by a subgroup of phages as a receptor. Using L. lactis and phage 1358 as a model, we investigated the interaction between the phage RBP and the pellicle hexasaccharide of the host strain. A core trisaccharide (TriS), derived from the pellicle hexasaccharide repeating unit, was chemically synthesised, and the crystal structure of the RBP/TriS complex was determined. This provided unprecedented structural details of RBP/receptor site-specific binding. The complete hexasaccharide repeating unit was modelled and found to aptly fit the extended binding site. The specificity observed in in vivo phage adhesion assays could be interpreted in view of the reported structure. Therefore, by combining synthetic carbohydrate chemistry, X-ray crystallography and phage plaquing assays, we suggest that phage adsorption results from distinct recognition of the RBP towards the core TriS or the remaining residues of the hexasacchride receptor. This study provides a novel insight into the adsorption process of phages targeting saccharides as their receptors.

PubMed: 25708888
DOI: 10.1111/mmi.12978

実験手法

X-RAY DIFFRACTION (2.1 Å)