4RCD

Crystal structure of BACE1 in complex with a 2-aminooxazoline 4-azaxanthene inhibitor

4RCD の概要

• エントリーDOI: 10.2210/pdb4rcd/pdb
• 関連するPDBエントリー: 4RCE 4RCF
• 分子名称: Beta-secretase 1, (5S)-7-(2-fluoropyridin-3-yl)-3-[(3-methyloxetan-3-yl)ethynyl]spiro[chromeno[2,3-b]pyridine-5,4'-[1,3]oxazol]-2'-amine, IODIDE ION, ... (5 entities in total)
• 機能のキーワード: aspartyl protease, alzheimer's disease, app, amyloid precursor protein, brain, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P56817
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46829.79

構造登録者

Whittington, D.A.,Long, A.M. (登録日: 2014-09-15, 公開日: 2015-01-21, 最終更新日: 2024-11-06)

主引用文献

Dineen, T.A.,Chen, K.,Cheng, A.C.,Derakhchan, K.,Epstein, O.,Esmay, J.,Hickman, D.,Kreiman, C.E.,Marx, I.E.,Wahl, R.C.,Wen, P.H.,Weiss, M.M.,Whittington, D.A.,Wood, S.,Fremeau, R.T.,White, R.D.,Patel, V.F.
Inhibitors of beta-Site Amyloid Precursor Protein Cleaving Enzyme (BACE1): Identification of (S)-7-(2-Fluoropyridin-3-yl)-3-((3-methyloxetan-3-yl)ethynyl)-5'H-spiro[chromeno[2,3-b]pyridine-5,4'-oxazol]-2'-amine (AMG-8718).
J.Med.Chem., 57:9811-9831, 2014

PubMed Abstract: We have previously shown that the aminooxazoline xanthene scaffold can generate potent and orally efficacious BACE1 inhibitors although certain of these compounds exhibited potential hERG liabilities. In this article, we describe 4-aza substitution on the xanthene core as a means to increase BACE1 potency while reducing hERG binding affinity. Further optimization of the P3 and P2' side chains resulted in the identification of 42 (AMG-8718), a compound with a balanced profile of BACE1 potency, hERG binding affinity, and Pgp recognition. This compound produced robust and sustained reductions of CSF and brain Aβ levels in a rat pharmacodynamic model and exhibited significantly reduced potential for QTc elongation in a cardiovascular safety model.

PubMed: 25363711
DOI: 10.1021/jm5012676

実験手法

X-RAY DIFFRACTION (1.9 Å)