4R8T

Structure of JEV protease

4R8T の概要

• エントリーDOI: 10.2210/pdb4r8t/pdb
• 分子名称: Serine protease subunit NS2B, NS3, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: serine protease, protease, ns2b, hydrolase
• 由来する生物種: Japanese encephalitis virus; 詳細
• 細胞内の位置: Capsid protein C: Virion . Peptide pr: Secreted . Small envelope protein M: Virion membrane ; Multi-pass membrane protein . Envelope protein E: Virion membrane ; Multi-pass membrane protein . Non-structural protein 1: Secreted. Non-structural protein 2A: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Serine protease subunit NS2B: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Serine protease NS3: Host endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side : P14403
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 17687.26

構造登録者

Nair, D.T.,Weinert, T.,Wang, M.,Olieric, V. (登録日: 2014-09-03, 公開日: 2014-12-24, 最終更新日: 2024-03-20)

主引用文献

Weinert, T.,Olieric, V.,Waltersperger, S.,Panepucci, E.,Chen, L.,Zhang, H.,Zhou, D.,Rose, J.,Ebihara, A.,Kuramitsu, S.,Li, D.,Howe, N.,Schnapp, G.,Pautsch, A.,Bargsten, K.,Prota, A.E.,Surana, P.,Kottur, J.,Nair, D.T.,Basilico, F.,Cecatiello, V.,Pasqualato, S.,Boland, A.,Weichenrieder, O.,Wang, B.C.,Steinmetz, M.O.,Caffrey, M.,Wang, M.
Fast native-SAD phasing for routine macromolecular structure determination.
Nat.Methods, 12:131-133, 2015

PubMed Abstract: We describe a data collection method that uses a single crystal to solve X-ray structures by native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real-life examples, including a human membrane protein, a protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this method. The data collection strategy is suitable for routine structure determination and can be implemented at most macromolecular crystallography synchrotron beamlines.

PubMed: 25506719
DOI: 10.1038/nmeth.3211

実験手法

X-RAY DIFFRACTION (2.133 Å)