4R75

Structure of the periplasmic binding protein AfuA from Actinobacillus pleuropneumoniae (exogenous sedoheptulose-7-phosphate bound)

4R75 の概要

• エントリーDOI: 10.2210/pdb4r75/pdb
• 関連するPDBエントリー: 4R72 4R73 4R74
• 分子名称: ABC-type Fe3+ transport system, periplasmic component, 1-C-(hydroxymethyl)-6-O-phosphono-beta-D-altrofuranose, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: abc transporter, sugar transporter, glucose-6-phosphate, fructose-6-phosphate, sedoheptulose-7-phosphate, transport protein
• 由来する生物種: Actinobacillus pleuropneumoniae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36108.60

構造登録者

Sit, B.,Calmettes, C.,Moraes, T.F. (登録日: 2014-08-26, 公開日: 2015-08-12, 最終更新日: 2024-11-20)

主引用文献

Sit, B.,Crowley, S.M.,Bhullar, K.,Lai, C.C.,Tang, C.,Hooda, Y.,Calmettes, C.,Khambati, H.,Ma, C.,Brumell, J.H.,Schryvers, A.B.,Vallance, B.A.,Moraes, T.F.
Active Transport of Phosphorylated Carbohydrates Promotes Intestinal Colonization and Transmission of a Bacterial Pathogen.
Plos Pathog., 11:e1005107-e1005107, 2015

PubMed Abstract: Efficient acquisition of extracellular nutrients is essential for bacterial pathogenesis, however the identities and mechanisms for transport of many of these substrates remain unclear. Here, we investigate the predicted iron-binding transporter AfuABC and its role in bacterial pathogenesis in vivo. By crystallographic, biophysical and in vivo approaches, we show that AfuABC is in fact a cyclic hexose/heptose-phosphate transporter with high selectivity and specificity for a set of ubiquitous metabolites (glucose-6-phosphate, fructose-6-phosphate and sedoheptulose-7-phosphate). AfuABC is conserved across a wide range of bacterial genera, including the enteric pathogens EHEC O157:H7 and its murine-specific relative Citrobacter rodentium, where it lies adjacent to genes implicated in sugar sensing and acquisition. C. rodentium ΔafuA was significantly impaired in an in vivo murine competitive assay as well as its ability to transmit infection from an afflicted to a naïve murine host. Sugar-phosphates were present in normal and infected intestinal mucus and stool samples, indicating that these metabolites are available within the intestinal lumen for enteric bacteria to import during infection. Our study shows that AfuABC-dependent uptake of sugar-phosphates plays a critical role during enteric bacterial infection and uncovers previously unrecognized roles for these metabolites as important contributors to successful pathogenesis.

PubMed: 26295949
DOI: 10.1371/journal.ppat.1005107

実験手法

X-RAY DIFFRACTION (1.278 Å)