4R49

Racemic crystal structure of a calcium-bound B-DNA duplex

4R49 の概要

• エントリーDOI: 10.2210/pdb4r49/pdb
• 関連するPDBエントリー: 4R44 4R45 4R47 4R48 4R4A 4R4D
• 分子名称: 5'-D(*CP*CP*GP*GP*TP*AP*CP*CP*GP*G)-3', CALCIUM ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: racemic dna, racemates, dna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6418.51

構造登録者

Mandal, P.K.,Collie, G.W.,Kauffmann, B.,Huc, I. (登録日: 2014-08-19, 公開日: 2014-11-12, 最終更新日: 2023-09-20)

主引用文献

Mandal, P.K.,Collie, G.W.,Kauffmann, B.,Huc, I.
Racemic DNA crystallography.
Angew.Chem.Int.Ed.Engl., 53:14424-14427, 2014

PubMed Abstract: Racemates increase the chances of crystallization by allowing molecular contacts to be formed in a greater number of ways. With the advent of protein synthesis, the production of protein racemates and racemic-protein crystallography are now possible. Curiously, racemic DNA crystallography had not been investigated despite the commercial availability of L- and D-deoxyribo-oligonucleotides. Here, we report a study into racemic DNA crystallography showing the strong propensity of racemic DNA mixtures to form racemic crystals. We describe racemic crystal structures of various DNA sequences and folded conformations, including duplexes, quadruplexes, and a four-way junction, showing that the advantages of racemic crystallography should extend to DNA.

PubMed: 25358289
DOI: 10.1002/anie.201409014

実験手法

X-RAY DIFFRACTION (1.28 Å)