4R44

Racemic crystal structure of a tetramolecular DNA G-quadruplex

Summary for 4R44

• Entry DOI: 10.2210/pdb4r44/pdb
• Related: 4R45 4R47 4R48 4R49 4R4A 4R4D
• Descriptor: 5'-D(*TP*GP*GP*GP*GP*T)-3', POTASSIUM ION, SODIUM ION, ... (4 entities in total)
• Functional Keywords: racemic dna, racemates, dna
• Biological source: synthetic construct
• Total number of polymer chains: 16
• Total formula weight: 30723.35

Authors

Mandal, P.K.,Collie, G.W.,Kauffmann, B.,Huc, I. (deposition date: 2014-08-19, release date: 2014-11-12, Last modification date: 2023-09-20)

Primary citation

Mandal, P.K.,Collie, G.W.,Kauffmann, B.,Huc, I.
Racemic DNA crystallography.
Angew.Chem.Int.Ed.Engl., 53:14424-14427, 2014

PubMed Abstract: Racemates increase the chances of crystallization by allowing molecular contacts to be formed in a greater number of ways. With the advent of protein synthesis, the production of protein racemates and racemic-protein crystallography are now possible. Curiously, racemic DNA crystallography had not been investigated despite the commercial availability of L- and D-deoxyribo-oligonucleotides. Here, we report a study into racemic DNA crystallography showing the strong propensity of racemic DNA mixtures to form racemic crystals. We describe racemic crystal structures of various DNA sequences and folded conformations, including duplexes, quadruplexes, and a four-way junction, showing that the advantages of racemic crystallography should extend to DNA.

PubMed: 25358289
DOI: 10.1002/anie.201409014

Experimental method

X-RAY DIFFRACTION (2.695 Å)