4R3H

The crystal structure of an apo RNA binding protein

Summary for 4R3H

• Entry DOI: 10.2210/pdb4r3h/pdb
• Related: 4R3I
• Descriptor: YTH domain-containing protein 1, UNKNOWN ATOM OR ION, SULFATE ION, ... (4 entities in total)
• Functional Keywords: structural genomics, structural genomics consortium, sgc, rna binding protein
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus : Q96MU7
• Total number of polymer chains: 2
• Total formula weight: 37743.72

Authors

Xu, C.,Liu, K.,Tempel, W.,Li, Y.,Bountra, C.,Arrowsmith, C.H.,Edwards, A.M.,Min, J.,Structural Genomics Consortium (SGC) (deposition date: 2014-08-15, release date: 2014-09-17, Last modification date: 2024-02-28)

Primary citation

Xu, C.,Wang, X.,Liu, K.,Roundtree, I.A.,Tempel, W.,Li, Y.,Lu, Z.,He, C.,Min, J.
Structural basis for selective binding of m(6)A RNA by the YTHDC1 YTH domain.
Nat.Chem.Biol., 10:927-929, 2014

PubMed Abstract: N(6)-methyladenosine (m(6)A) is the most abundant internal modification of nearly all eukaryotic mRNAs and has recently been reported to be recognized by the YTH domain family proteins. Here we present the crystal structures of the YTH domain of YTHDC1, a member of the YTH domain family, and its complex with an m(6)A-containing RNA. Our structural studies, together with transcriptome-wide identification of YTHDC1-binding sites and biochemical experiments, not only reveal the specific mode of m(6)A-YTH binding but also explain the preferential recognition of the GG(m(6)A)C sequences by YTHDC1.

PubMed: 25242552
DOI: 10.1038/nchembio.1654

Experimental method

X-RAY DIFFRACTION (1.9 Å)