4R3H

The crystal structure of an apo RNA binding protein

4R3H の概要

• エントリーDOI: 10.2210/pdb4r3h/pdb
• 関連するPDBエントリー: 4R3I
• 分子名称: YTH domain-containing protein 1, UNKNOWN ATOM OR ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: structural genomics, structural genomics consortium, sgc, rna binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus : Q96MU7
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37743.72

構造登録者

Xu, C.,Liu, K.,Tempel, W.,Li, Y.,Bountra, C.,Arrowsmith, C.H.,Edwards, A.M.,Min, J.,Structural Genomics Consortium (SGC) (登録日: 2014-08-15, 公開日: 2014-09-17, 最終更新日: 2024-02-28)

主引用文献

Xu, C.,Wang, X.,Liu, K.,Roundtree, I.A.,Tempel, W.,Li, Y.,Lu, Z.,He, C.,Min, J.
Structural basis for selective binding of m(6)A RNA by the YTHDC1 YTH domain.
Nat.Chem.Biol., 10:927-929, 2014

PubMed Abstract: N(6)-methyladenosine (m(6)A) is the most abundant internal modification of nearly all eukaryotic mRNAs and has recently been reported to be recognized by the YTH domain family proteins. Here we present the crystal structures of the YTH domain of YTHDC1, a member of the YTH domain family, and its complex with an m(6)A-containing RNA. Our structural studies, together with transcriptome-wide identification of YTHDC1-binding sites and biochemical experiments, not only reveal the specific mode of m(6)A-YTH binding but also explain the preferential recognition of the GG(m(6)A)C sequences by YTHDC1.

PubMed: 25242552
DOI: 10.1038/nchembio.1654

実験手法

X-RAY DIFFRACTION (1.9 Å)