4QYS

TrpB2 enzymes

4QYS の概要

• エントリーDOI: 10.2210/pdb4qys/pdb
• 分子名称: Tryptophan synthase beta chain 2, (5-HYDROXY-4,6-DIMETHYLPYRIDIN-3-YL)METHYL DIHYDROGEN PHOSPHATE, PYRIDOXAL-5'-PHOSPHATE, ... (5 entities in total)
• 機能のキーワード: enzyme evolution, functional annotation, tryptophan synthase, lyase
• 由来する生物種: Sulfolobus solfataricus P2
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 99394.64

構造登録者

Busch, F.,Rajendran, C.,Loeffler, P.,Merkl, R.,Sterner, R. (登録日: 2014-07-25, 公開日: 2015-02-18, 最終更新日: 2024-03-20)

主引用文献

Busch, F.,Rajendran, C.,Mayans, O.,Loffler, P.,Merkl, R.,Sterner, R.
TrpB2 enzymes are O-phospho-l-serine dependent tryptophan synthases
Biochemistry, 53:6078-6083, 2014

PubMed Abstract: The rapid increase of the number of sequenced genomes asks for the functional annotation of the encoded enzymes. We used a combined computational-structural approach to determine the function of the TrpB2 subgroup of the tryptophan synthase β chain/β chain-like TrpB1-TrpB2 family (IPR023026). The results showed that TrpB2 enzymes are O-phospho-l-serine dependent tryptophan synthases, whereas TrpB1 enzymes catalyze the l-serine dependent synthesis of tryptophan. We found a single residue being responsible for the different substrate specificities of TrpB1 and TrpB2 and confirmed this finding by mutagenesis studies and crystallographic analysis of a TrpB2 enzyme with bound O-phospho-l-serine.

PubMed: 25184516
DOI: 10.1021/bi500977y

実験手法

X-RAY DIFFRACTION (1.939 Å)