4QY9

X-ray structure of the adduct between hen egg white lysozyme and Auoxo3, a cytotoxic gold(III) compound

Summary for 4QY9

• Entry DOI: 10.2210/pdb4qy9/pdb
• Descriptor: Lysozyme C, NITRATE ION, 1,2-ETHANEDIOL, ... (6 entities in total)
• Functional Keywords: hydrolase
• Biological source: Gallus gallus (chicken)
• Cellular location: Secreted: P00698
• Total number of polymer chains: 1
• Total formula weight: 15120.24

Authors

Russo Krauss, I.,Merlino, A. (deposition date: 2014-07-24, release date: 2014-11-05, Last modification date: 2014-11-19)

Primary citation

Russo Krauss, I.,Messori, L.,Cinellu, M.A.,Marasco, D.,Sirignano, R.,Merlino, A.
Interactions of gold-based drugs with proteins: the structure and stability of the adduct formed in the reaction between lysozyme and the cytotoxic gold(iii) compound Auoxo3.
Dalton Trans, 43:17483-17488, 2014

PubMed Abstract: The structure and stability of the adduct formed in the reaction between Auoxo3, a dinuclear gold(iii) compound, and the model protein hen egg white lysozyme (HEWL) are investigated by X-ray crystallography, UV-Vis absorption spectroscopy and circular dichroism (CD). It is found that Auoxo3 breaks down completely, undergoes reduction and produces reactive gold(i) species able to bind the protein and form stable derivatives. The behaviour of Auoxo3 is compared with that of two analogous gold(iii) complexes previously studied: a few significant differences are highlighted. The general implications of these new results for the mode of action of cytotoxic gold complexes are discussed.

PubMed: 25340580
DOI: 10.1039/c4dt02332c

Experimental method

X-RAY DIFFRACTION (2.05 Å)