4QX0

Cry3A Toxin structure obtained by Serial Femtosecond Crystallography from in vivo grown crystals isolated from Bacillus thuringiensis and data processed with the cctbx.xfel software suite

4QX0 の概要

• エントリーDOI: 10.2210/pdb4qx0/pdb
• 関連するPDBエントリー: 4QX1 4QX2 4QX3
• 分子名称: Pesticidal crystal protein cry3Aa (2 entities in total)
• 機能のキーワード: in vivo crystals, microcrystals, serial femtosecond crystallography, sfx, lcls, x-ray free-electron laser, insecticidal toxin, toxin
• 由来する生物種: Bacillus thuringiensis serovar tenebrionis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 66602.04

構造登録者

Sawaya, M.R.,Cascio, D.,Gingery, M.,Rodriguez, J.,Goldschmidt, L.,Colletier, J.-P.,Messerschmidt, M.,Boutet, S.,Koglin, J.E.,Williams, G.J.,Brewster, A.S.,Nass, K.,Hattne, J.,Botha, S.,Doak, R.B.,Shoeman, R.L.,DePonte, D.P.,Park, H.-W.,Federici, B.A.,Sauter, N.K.,Schlichting, I.,Eisenberg, D. (登録日: 2014-07-17, 公開日: 2014-08-13, 最終更新日: 2024-02-28)

主引用文献

Sawaya, M.R.,Cascio, D.,Gingery, M.,Rodriguez, J.,Goldschmidt, L.,Colletier, J.P.,Messerschmidt, M.M.,Boutet, S.,Koglin, J.E.,Williams, G.J.,Brewster, A.S.,Nass, K.,Hattne, J.,Botha, S.,Doak, R.B.,Shoeman, R.L.,DePonte, D.P.,Park, H.W.,Federici, B.A.,Sauter, N.K.,Schlichting, I.,Eisenberg, D.S.
Protein crystal structure obtained at 2.9 angstrom resolution from injecting bacterial cells into an X-ray free-electron laser beam.
Proc.Natl.Acad.Sci.USA, 111:12769-12774, 2014

PubMed Abstract: It has long been known that toxins produced by Bacillus thuringiensis (Bt) are stored in the bacterial cells in crystalline form. Here we describe the structure determination of the Cry3A toxin found naturally crystallized within Bt cells. When whole Bt cells were streamed into an X-ray free-electron laser beam we found that scattering from other cell components did not obscure diffraction from the crystals. The resolution limits of the best diffraction images collected from cells were the same as from isolated crystals. The integrity of the cells at the moment of diffraction is unclear; however, given the short time (∼ 5 µs) between exiting the injector to intersecting with the X-ray beam, our result is a 2.9-Å-resolution structure of a crystalline protein as it exists in a living cell. The study suggests that authentic in vivo diffraction studies can produce atomic-level structural information.

PubMed: 25136092
DOI: 10.1073/pnas.1413456111

実験手法

X-RAY DIFFRACTION (2.8 Å)