4QTQ

Structure of a Xanthomonas Type IV Secretion System related protein

4QTQ の概要

• エントリーDOI: 10.2210/pdb4qtq/pdb
• 分子名称: XAC2610 protein, CALCIUM ION (3 entities in total)
• 機能のキーワード: beta-sandwich, calcium binding motif, beta-propeller fragment, peptidoglycan hydrolase inhibitor, immunity protein xanthomonas, hydrolase inhibitor
• 由来する生物種: Xanthomonas axonopodis pv. citri
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23357.08

構造登録者

Souza, D.P.,Guzzo, C.R.,Farah, C.S. (登録日: 2014-07-08, 公開日: 2015-06-17, 最終更新日: 2024-10-09)

主引用文献

Souza, D.P.,Oka, G.U.,Alvarez-Martinez, C.E.,Bisson-Filho, A.W.,Dunger, G.,Hobeika, L.,Cavalcante, N.S.,Alegria, M.C.,Barbosa, L.R.,Salinas, R.K.,Guzzo, C.R.,Farah, C.S.
Bacterial killing via a type IV secretion system.
Nat Commun, 6:6453-6453, 2015

PubMed Abstract: Type IV secretion systems (T4SSs) are multiprotein complexes that transport effector proteins and protein-DNA complexes through bacterial membranes to the extracellular milieu or directly into the cytoplasm of other cells. Many bacteria of the family Xanthomonadaceae, which occupy diverse environmental niches, carry a T4SS with unknown function but with several characteristics that distinguishes it from other T4SSs. Here we show that the Xanthomonas citri T4SS provides these cells the capacity to kill other Gram-negative bacterial species in a contact-dependent manner. The secretion of one type IV bacterial effector protein is shown to require a conserved C-terminal domain and its bacteriolytic activity is neutralized by a cognate immunity protein whose 3D structure is similar to peptidoglycan hydrolase inhibitors. This is the first demonstration of the involvement of a T4SS in bacterial killing and points to this special class of T4SS as a mediator of both antagonistic and cooperative interbacterial interactions.

PubMed: 25743609
DOI: 10.1038/ncomms7453

実験手法

X-RAY DIFFRACTION (2 Å)