4QBY

yCP in complex with BOC-ALA-ALA-ALA-CHO

4QBY の概要

• エントリーDOI: 10.2210/pdb4qby/pdb
• 関連するPDBエントリー: 1RYP
• 関連するBIRD辞書のPRD_ID: PRD_001237
• 分子名称: Proteasome subunit alpha type-2, Proteasome subunit beta type-4, Proteasome subunit beta type-5, ... (17 entities in total)
• 機能のキーワード: 20s proteasome, peptide aldehyde, allosteric regulation, pca analysis, immunoproteasome, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Cytoplasm: P23639 P22141 P30656 P23724 P30657 P38624 P23638 P40303 P32379 P40302 P21242 P21243 P25043 P25451
• タンパク質・核酸の鎖数: 32
• 化学式量合計: 732587.65

構造登録者

Arciniega, M.,Beck, P.,Lange, O.,Groll, M.,Huber, R. (登録日: 2014-05-09, 公開日: 2014-06-18, 最終更新日: 2023-11-15)

主引用文献

Arciniega, M.,Beck, P.,Lange, O.F.,Groll, M.,Huber, R.
Differential global structural changes in the core particle of yeast and mouse proteasome induced by ligand binding.
Proc.Natl.Acad.Sci.USA, 111:9479-9484, 2014

PubMed Abstract: Two clusters of configurations of the main proteolytic subunit β5 were identified by principal component analysis of crystal structures of the yeast proteasome core particle (yCP). The apo-cluster encompasses unliganded species and complexes with nonpeptidic ligands, and the pep-cluster comprises complexes with peptidic ligands. The murine constitutive CP structures conform to the yeast system, with the apo-form settled in the apo-cluster and the PR-957 (a peptidic ligand) complex in the pep-cluster. In striking contrast, the murine immune CP classifies into the pep-cluster in both the apo and the PR-957-liganded species. The two clusters differ essentially by multiple small structural changes and a domain motion enabling enclosure of the peptidic ligand and formation of specific hydrogen bonds in the pep-cluster. The immune CP species is in optimal peptide binding configuration also in its apo form. This favors productive ligand binding and may help to explain the generally increased functional activity of the immunoproteasome. Molecular dynamics simulations of the representative murine species are consistent with the experimentally observed configurations. A comparison of all 28 subunits of the unliganded species with the peptidic liganded forms demonstrates a greatly enhanced plasticity of β5 and suggests specific signaling pathways to other subunits.

PubMed: 24979800
DOI: 10.1073/pnas.1408018111

実験手法

X-RAY DIFFRACTION (3 Å)